| 实时定量PCR的原理及其在植物病理学研究中的应用 |
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| 引用本文： | 吴静,吴茂森,何晨阳.实时定量PCR的原理及其在植物病理学研究中的应用[J].植物保护,2007,33(6):123-128. |
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| 作者姓名： | 吴静 吴茂森 何晨阳 |
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| 作者单位： | 中国农业科学院植物保护研究所 北京100094 |
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| 摘 要： | 实时定量PCR(Real-time quantitative)是一种利用荧光信号实时监测每个循环的扩增产物,从而实现对模板进行定量分析的新技术。它具有快速、灵敏、精确等优点。该技术在分子诊断、分子生物学研究、动植物检疫以及食品安全检测等方面有广泛的应用。本文综述了实时定量PCR的原理、影响因素及其在植物病理学研究中的应用。
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| 关 键 词： | 实时定量PCR Ct值 定量分析 应用 |
| 收稿时间： | 2007-03-09 |
| 修稿时间： | 2007-04-10 |
Principles of real-time quantitative PCR and its application in plant pathology |
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| Wu Jing;Wu Maosen;He Chenyang.Principles of real-time quantitative PCR and its application in plant pathology[J].Plant Protection,2007,33(6):123-128. |
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| Authors: | Wu Jing;Wu Maosen;He Chenyang |
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| Institution: | Institute of Plant Protection;CAAS;Beijing 100094;China |
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| Abstract: | Real-time quantitative PCR(polymerase chain reaction) is a new method used to analyze the template concentration by monitoring the fluorescent signal during each amplification cycles.Benefits of this technology over conventional ones include high sensitivity,large dynamic range and accurate quantification.It is widely used in molecular diagnostics,molecular biology research and animal and plant quarantine,etc.In this review,the principles,key factors of this method and its application in plant pathology were discussed. |
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| Keywords: | real-time quantitative PCR Ct Value quantitative analysis application |
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