32个银杏品种的RAPD遗传多态性及其分类

以32个银杏(Ginkgo bilobaL.)品种的鲜叶为材料,提取其总DNA,从56个随机引物中筛选出8个单引物和4对双引物进行RAPD扩增.以随机扩增多态性DNA(RAPD)标记方法进行遗传多样性分析,用NTSYS-pc2.10e软件计算样品间的Dice相似性系数,并按UPGMA法进行了聚类分析.结果表明,8个单引物和4对双引物扩增共得到85个位点,其中81个(95%)是多态性的,说明供试样品在DNA水平上有很高的遗传多样性.32个供试的样品可分为两大类,北京梅核、南雄上矽(雄)、华口大白果为一类,其它的为第二类.在四个泰兴品种中泰兴2号和泰兴3号的相似性系数为0.968,表明它们的遗传关系比较近.本研究在分子生物学方面为银杏的品种分类和遗传多样性提供了一些新依据.

Genetic diversity analysis of 32 Ginkgo varieties by RAPD

The leaves of 32 ginkgo cultivars(Ginkgo biloba L.) were used to extract genomic DNA.8 single primers and 4 primer pairs were selected from 56 random primers for RAPD amplification.RAPD amplification on agarose gel was used for studies on genetic diversity and NTSYS-pc 2.10e software was used to calculate the Dice similarity coefficient,and dendrogram was constructed using UPGMA method based on Dice similarity coefficient.The results showed that 85 amplified loci were detected,of which 81(95%) were polymorphic.It has been proved that the 32 samples presented much higher genetic diversity on DNA molecular level.They may be divided into 2 groups.The cultivars including Beijing-meihe,Nanxiong-shangdai,Huakou-dabaiguo were in one group,and the others were in another group.In the four Taixing cultivars,Taixing No.2 and Taixing No.3 have 0.968 average similarities,showing that they were low genetic diversity and implying that they may belong to the same cultivars.