| 鸡传染性支气管炎病毒单克隆抗体的制备及鉴定 |
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| 引用本文： | 姜亦飞,亓丽红,黄兵,李玉峰,于可响,宋敏训. 鸡传染性支气管炎病毒单克隆抗体的制备及鉴定[J]. 山东农业科学, 2011, 0(1): 100-102 |
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| 作者姓名： | 姜亦飞 亓丽红 黄兵 李玉峰 于可响 宋敏训 |
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| 作者单位： | 山东省农业科学院家禽研究所/山东省禽病诊断与免疫重点实验室,山东,济南,250023 |
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| 基金项目： | 国家科技支撑计划，山东省农业科学院青年基金 |
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| 摘 要： | 用纯化的鸡传染性支气管炎病毒免疫BALB／c小鼠，取其脾细胞与骨髓瘤细胞Ag8．653融合。对杂交瘤细胞及时筛选，阳性孔经3次有限稀释法克隆，成功获得3株能稳定传代并分泌抗鸡传染性支气管炎病毒单克隆抗体的杂交瘤细胞株1F4F7D9、3E9G3D4、48883F8。通过间接ELISA、血凝抑制以及琼脂糖扩散等方法检测，3株单克隆抗体的腹水间接ELISA效价达10^6以上并且具有良好的特异性。
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| 关 键 词： | 鸡传染性支气管炎病毒 单克隆抗体 制备 鉴定 |
Preparation and Identification of Monoclonal Antibody of Avian Infectious Bronchitis Virus |
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| JIANG Yi-fei,QI Li-hong,HUANG Bing,LI Yu-feng,YU Ke-xiang,SONG Min-xun. Preparation and Identification of Monoclonal Antibody of Avian Infectious Bronchitis Virus[J]. Shandong Agricultural Sciences, 2011, 0(1): 100-102 |
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| Authors: | JIANG Yi-fei QI Li-hong HUANG Bing LI Yu-feng YU Ke-xiang SONG Min-xun |
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| Affiliation: | (Poultry Institute of Shandong Academy of Agricultural Sciences/ Shandong Key Laboratory of Avian Disease Diagnosis and Immunization,Jinan 250023,China) |
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| Abstract: | BALB/c mice were regularly immunized with purified avian infectious bronchitis virus(IBV).Their spleen cells were collected and infused with myeloma cell Ag8.653.After selection in time and 3 times clone by limiting dilution,3 hybridoma cell lines against IBV named 1F4F7D9,3E9G3D4 and 4B8B3F8 were obtained.The indirect ELISA titers of their ascites were all above 106 by indirect ELISA detection,hemagglutination inhibition and agar gel precipitin test.It was proved that 3 cell lines all possessed the favourable specificity. |
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| Keywords: | Avian infectious bronchitis virus(IBV) Monoclonal antibody Preparation Identification |
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