转TaLEA基因小黑杨抗寒株系的筛选

为验证转TaLEA基因小黑杨耐低温能力并筛选抗寒的优良株系,对11个转LEA基因小黑杨株系与非转基因野生型小黑杨对照(WT)进行了低温胁迫处理,测定其丙二醛(MDA)的质量摩尔浓度、过氧化物酶( POD)活性、过氧化氢酶(CAT)活性、叶绿素质量分数、冷害指数等.结果表明:低温胁迫条件下,除XL09外,其余各转基因株系...

Selection of Cold Resistant Strains from TaLEA Gene Transferred Populus simonii ×P.nigra

An experiment was conducted to study the activities of POD and CAT,contents of MDA and chlorophyll as well as Chilling Injury Index(CI index) between eleven clones of transgenic Populus simonii × P.nigra with TaLEA(late-embryogenesis-abundant protein gene from Tamatix sp.) and non-transgenic ones(WT) in order to examine the cold tolerance of woody plants.Results indicated that the MDA content of each transgenic clone was lower than that of WT except XL09,and the average MDA content of the eleven transgenic clones was 16.5 % lower than that of WT.In contrast to WT,the activities of anti-oxidative enzymes in XL10 and XL11 exhibited relatively higher,while those of the others showed relatively lower.Chlorophyll content of each transgenic clone was superior to that of WT except XL01,and the average chlorophyll content of the eleven transgenic clones was 35.1mg·g-1,which was 6.4% lower than that of WT.Moreover,compared with WT,lower values of CI index,except in XL03,were observed in all transgenic poplar plants.The cold resistance of XL11,assessed by membership function,was the strongest,followed by XL10,XL14,XL04,XL07,XL03,XL13,WT,XL06,XL01,XL05 and XL09.These results suggest that the introduction of external LEA to poplar plants may enhance the cold tolerance of the clones,especially for XL11,XL10,XL14 and XL04 clones in this study.