| 萝藦的组织培养与植株再生研究 |
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| 引用本文： | 郑宏丽,宋佳,刘羽,邹翠霞,姜长阳.萝藦的组织培养与植株再生研究[J].湖南农业科学,2009(1). |
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| 作者姓名： | 郑宏丽 宋佳 刘羽 邹翠霞 姜长阳 |
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| 作者单位： | 辽宁师范大学生命科学学院,辽宁大连,116029 |
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| 摘 要： | 以具有有利变异性状萝藦茎上的生长点为外植体,以不同浓度的BA、IAA、NAA、2,4-D、GA的MS、1/2MS和1/3 MS为基本培养基,进行了芽伸长生长培养、芽分化培养和试管苗生根培养研究.结果表明:1/2 MS+IAA 0.2mg/L是促进萝藦培养芽伸长生长的理想培养基;MS+BA 0.5 mg/L+NAA 0.1 mg/L+GA 0.5 ms/L是萝藦芽分化培养的理想培养基;1/3 MS+IAA 0.5 mg/L是萝藦试管苗生根培养的理想培养基.
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| 关 键 词： | 萝藦 组织培养 愈伤组织 植株再生 |
Study on Tissue Culture and Plant Regeneration of Metaplexis Japonica |
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| ZHENG Hong-li,SONG Jia,LIU Yu,ZOU Cui-xia,JIANG Chang-yang.Study on Tissue Culture and Plant Regeneration of Metaplexis Japonica[J].Hunan Agricultural Sciences,2009(1). |
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| Authors: | ZHENG Hong-li SONG Jia LIU Yu ZOU Cui-xia JIANG Chang-yang |
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| Institution: | Biology Scientific College;Liaoning Normal University;Dalian 116029;PRC |
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| Abstract: | Growing points of metaplexis japonica roots were used as explants, and different concentrations of BA, IAA, NAA, 2,4-D, GA, and MS, 1/2 MS or 1/3 MS as the basic culture medium. Bud elongation culture, bud differentiation culture and rooting of vitro seedling were carried out. The results showed that 1/2 MS + IAA 0.2 mg/L was good culture medium for promoting bud of metaplexis japonica elongating and growing; MS+BA 0.5 mg/L+NAA 0.1 mg/L+GA 0.5 mg/L was good for bud differentiation; and 1/3 MS+IAA 0.5 mg/L w... |
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| Keywords: | Metaplexis japonica tissue culture callus plant regeneration |
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