| 不同冷冻方法对GV期绵羊卵母细胞发育效果的影响 |
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| 引用本文： | 周佰成.不同冷冻方法对GV期绵羊卵母细胞发育效果的影响[J].中国草食动物,2009,29(6):3-4. |
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| 作者姓名： | 周佰成 |
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| 作者单位： | 青海省畜牧总站,西宁810001 |
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| 摘 要： | 卵母细胞冷冻保存具有广泛而潜在的应用价值。文章主要分析了不同冷冻方法对绵羊GV期卵母细胞发育效果的影响。GV期卵母细胞程序化冷冻解冻后形态正常率（54.8%）和体外培养成熟率（14.7%）均显著或极显著低于细管玻璃化冷冻（71.8%,29.5%;P〈0.05）和OPS玻璃化冷冻（78.3%,P〈0.01;35.4%,P〈0.05）;3种不同方法冷冻GV期卵母细胞成熟率与对照组相比均差异极显著（P〈0.01）。
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| 关 键 词： | GV期卵母细胞 冷冻 发育能力 绵羊 |
Effects of Different Freezing Methods on Developmental Capability of Ovine GV Stage Oocytes |
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| Zhou Bai-cheng.Effects of Different Freezing Methods on Developmental Capability of Ovine GV Stage Oocytes[J].China Herbivores,2009,29(6):3-4. |
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| Authors: | Zhou Bai-cheng |
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| Institution: | Zhou Bai-cheng (Qinghai General Station of Animal Husb,ry,Xining 810001) |
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| Abstract: | Oocytes cryopreservation is a potentially valuable technique for conservation of animal genetic resources.In this experiment,the influences of freezing methods on developmental capability of ovine GV stage oocytes were examined.Oocytes were frozen by programmed freezing,straw vitrification and open pulled straw vitrification(OPS) method,fresh oocytes served as the control.The results showed that,the rates of morphologically normal oocytes(54.8%) and the maturation rate(14.7%) with programmed freezing method... |
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| Keywords: | GV stage oocytes freezing developmental capacity ovine |
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