| 蝴蝶兰组织培养快速繁殖技术研究 |
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| 引用本文： | 朱志国,黄承钧,陶陶,闻治江.蝴蝶兰组织培养快速繁殖技术研究[J].安徽农业科学,2009,37(30):14614-14615. |
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| 作者姓名： | 朱志国 黄承钧 陶陶 闻治江 |
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| 作者单位： | 芜湖职业技术学院园林园艺系,安徽,芜湖,241000;芜湖职业技术学院园林园艺系,安徽,芜湖,241000;芜湖职业技术学院园林园艺系,安徽,芜湖,241000;芜湖职业技术学院园林园艺系,安徽,芜湖,241000 |
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| 基金项目： | 安徽省高校青年教师资助计划项目 |
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| 摘 要： | 目的]对蝴蝶兰的组织培养快速繁殖技术进行研究，为蝴蝶兰的批量生产提供参考。方法]以蝴蝶兰嫩叶、茎尖、花梗侧芽为外植体，进行蝴蝶兰原球茎诱导、增殖及试管苗生根的组织培养。结果]结果表明，MS＋2．0mg／L6-BA＋1．0ng／LNAA＋200ml／L椰乳是诱导蝴蝶兰原球茎形成的最佳培养基，茎尖诱导率迭缸．37％；MS＋1．0mg／LNAA＋2．0mg／L6-BA是蝴蝶兰原球茎增殖的最佳培养基，增殖系数达9．62；1／2MS是蝴蝶兰生根培养的最佳培养基，生根率达94．42％，且根生长最快最整齐。结论]在生产上，可采用试管苗生根继代培养的方法进行蝴蝶兰的快速繁殖。
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| 关 键 词： | 蝴蝶兰 组织培养 快速繁殖 |
Research on Tissue Culture and Rapid Propagation of Phalaenopsis amabilis |
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| ZHU Zhi-guo et al.Research on Tissue Culture and Rapid Propagation of Phalaenopsis amabilis[J].Journal of Anhui Agricultural Sciences,2009,37(30):14614-14615. |
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| Authors: | ZHU Zhi-guo |
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| Institution: | ZHU Zhi-guo et al (Department of L,scape Horticulture,Wuhu Institute of Occupation & Technology,Wuhu,Anhui 241000) |
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| Abstract: | Objective] The aim was to research the tissue culture and rapid propagation of Phalaenopsis amabilis,and provide reference for batch production of Phalaenopsis amabilis.Method] Taking tender leaves,the shoot-tips and pedicel lateral buds as explants,the conditions of original corm induction,proliferation and rooting of Phalaenopsis amabilis were discussed.Result] The result showed that the optimum medium for inducing the tender protocorm of Phalaenopsis amabilis was MS +2.0 mg/L 6-BA +1.0 mg/L NAA +200 m... |
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| Keywords: | Phalaenopsis amabilis Tissue culture Rapid propagation |
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