突变型抑癌基因p53在蛋鸡J亚群禽白血病中的表达

摘要: 蛋鸡J亚群禽白血病是病毒引起的肿瘤性传染病。为了探讨J亚群禽白血病病毒的致癌分子机理, 观察蛋用型鸡发生J亚群禽白血病时p53抑癌基因在各脏器中的表达, 应用链霉亲和素-生物素-过氧化物酶复合物免疫组化（SABC）法，研究了蛋用型鸡J亚群禽白血病自然病例的肝脏、肾脏、腺胃、肿瘤、输卵管、心脏、胰脏、骨髓、脾脏、肺脏、十二指肠、胸腺和法氏囊。结果表明：突变型p53在肝脏、肾脏、腺胃、肿瘤、输卵管中表达量最高，心脏、胰脏、骨髓、脾脏、肺脏、十二指肠、胸腺及法氏囊均可检出中等量的表达。这一结果提示突变型抑癌基因p53的表达参与蛋鸡J亚群禽白血病发生、发展过程。

Expression of Mutant Tumor Suppressor Gene p53 in Egg-type Chicken Infected with Avian Leukosis Viruse Subgroup J

Abstract：Avian leukosis in egg-type chickens is infectious neoplastic disease caused by avian leukosis viruse subgroup J( ALV-J). Expression of mutant tumor suppressor gene p53 in egg-type chicken with avian leukosis subgroup J was detected to study cancericidally molecular mechanism of ALV-J. Using immunohistochemistry method of strep avidin-biotin complex (SABC), many organs of illed egg-type chickens, involved liver, kidney, ventriculus glandularis, tumor, heart, pancreas, bone marrow, spleen, oviduct, lung, duodenum, thymus and bursa of fabricius were studied. And large numbers of mutant p53 protein were expressed in liver, kidney, ventriculus glandularis, tumor, oviduct. Moderate expression were detected in heart, pancreas, bone marrow, spleen, lung, duodenum, thymus and bursa of fabricius. The result suggested that expression of tumor suppressor gene p53 was concerned with occurrence and development of avian leukosis subgroup J.