| 片形吸虫Cold-SSCP鉴别方法的建立 |
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| 引用本文： | 邓国祥,张汉云,陈蓓蓉,李秋燕,林瑞庆,马妮妮,朱兴全. 片形吸虫Cold-SSCP鉴别方法的建立[J]. 中国畜牧兽医, 2007, 34(2): 86-88 |
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| 作者姓名： | 邓国祥 张汉云 陈蓓蓉 李秋燕 林瑞庆 马妮妮 朱兴全 |
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| 作者单位： | 1.华南农业大学兽医学院，广州 510642; 2.广州市番禺区市桥城区畜牧兽医站，广州 511400 |
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| 基金项目： | 国家自然科学基金;华南农业大学校科研和教改项目;华南农业大学校科研和教改项目 |
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| 摘 要： | 以采自我国不同地区的片形吸虫虫体为研究对象，PCR扩增出核糖体DNA (rDNA)的第一内转录间隔区(ITS-1)序列，然后采用非同位素的单链构象多态性(Cold-SSCP)方法分析PCR产物，对不同地区片形吸虫进行分子鉴定。所有样品经Cold-SSCP分析显示2种带型。样品测序及序列分析结果表明，第1种为肝片形吸虫带型，另1种为大片形吸虫带型。本研究建立了区分大片形吸虫和肝片形吸虫的Cold SSCP方法，可用于这2种吸虫的分子流行病学调查，从而为片形吸虫分子生物学的进一步研究奠定了基础。
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| 关 键 词： | 片形吸虫 rDNA ITS-1 Cold-SSCP |
| 文章编号： | 1671-7236（2007）02-0086-03 |
| 修稿时间： | 2006-07-24 |
Development of a Cold-SSCP Technique for the Identification of Fasciola Spp |
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| DENG Guo-xiang,ZHANG Han-yun,CHEN Bei-rong,LI Qiu-yan,LIN Rui-qing,MA Ni-ni,ZHU Xing-quan. Development of a Cold-SSCP Technique for the Identification of Fasciola Spp[J]. China Animal Husbandry & Veterinary Medicine, 2007, 34(2): 86-88 |
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| Authors: | DENG Guo-xiang ZHANG Han-yun CHEN Bei-rong LI Qiu-yan LIN Rui-qing MA Ni-ni ZHU Xing-quan |
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| Affiliation: | 1.College of Veterinary Medicine, South China Agricultural University，Guangzhou 510642，China; 2.Shiqiao Animal Husbandry and Veterinary Station, Panyu District, Guangzhou 511400, China |
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| Abstract: | A non-isotopic single strand conformation polymorphism(Cold-SSCP) technique was established to differentiate fasciola hepatica from fasciola gigantica from different geographical locations in China using the first internal transcribed spacers(ITS-1) of ribosomal DNA(rDNA) as genetic marker.The partial ITS-1 was amplified by PCR from individual fasciola and the amplicons were subjected to non-denaturing gel electrophoresis.Cold-SSCP analyses showed that fhepatica and fgigantica displayed distinct SSCP profiles,allowing their equivocal differentiation from each other.Sequencing analyses supported the results of Cold-SSCP.The establishment of the Cold-SSCP approach for the identification and differentiation of fhepatica and fgigantica has important implications for further studies of fasciola spp. |
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| Keywords: | rDNA ITS-1 Cold-SSCP |
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