| 不同营养元素及配比对油茶花粉萌发率的影响 |
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| 引用本文： | 袁德义,王瑞,袁军,廖婷,崔欣,蔡磊. 不同营养元素及配比对油茶花粉萌发率的影响[J]. 福建农林大学学报(自然科学版), 2010, 39(5) |
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| 作者姓名： | 袁德义 王瑞 袁军 廖婷 崔欣 蔡磊 |
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| 作者单位： | 1. 中南林业科技大学经济林育种与栽培国家林业局重点实验室,湖南,长沙,410004
2. 湖南省林业科学院,湖南,长沙,410004 |
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| 基金项目： | 国家“十一五”科技支撑计划资助项目(2009BADB1B0403;2009BADB1B0203); 林业公益性行业科研专项基金资助项目(200804044) |
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| 摘 要： | 以普通油茶花粉为试材,运用琼脂培养基萌发法研究N[CO(NH2)2]、P(KH2PO4)、K(KCl)、B(H3BO3)、Ca(CaCl2)、Mo(Na2Mo4)、Zn(ZnSO4)、Mg(MgCl2)等8种不同元素及其组合对油茶花粉萌发率的影响.结果表明,在单因素试验条件下,CaCl2、Na2Mo4、ZnSO4、KCl对油茶花粉萌发具有抑制作用,其中以ZnSO4的抑制作用最为明显;CO(NH2)2、KH2PO4、H3BO3、MgCl2对油茶花粉萌发具有明显的促进作用,4种元素最适宜浓度:CO(NH2)2为0.10-0.15 g.L-1;KH2PO4为0.05-0.10 g.L-1;H3BO3为0.10 g.L-1;MgCl2为0.05 g.L-1.正交试验结果表明,4种元素配合使用极显著地提高花粉萌发率,其中以0.15 g.L-1CO(NH2)2+0.15 g.L-1H3BO3+0.05 g.L-1MgCl2+0.05 g.L-1KH2PO4处理的效果最好,其花粉萌发率为90.51%,比CK提高了46.79%.
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| 关 键 词： | 油茶 营养元素 花粉 萌发率 |
The influence of nutrient elements on pollen germination percentage in Camellia oleifera |
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| YUAN De-yi,WANG Rui,YUAN Jun,LIAO Ting,CUI Xin,CAI Lei. The influence of nutrient elements on pollen germination percentage in Camellia oleifera[J]. Journal of Fujian Agricultural and Forestry University, 2010, 39(5) |
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| Authors: | YUAN De-yi WANG Rui YUAN Jun LIAO Ting CUI Xin CAI Lei |
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| Affiliation: | YUAN De-yi1,WANG Rui2,YUAN Jun1,LIAO Ting1,CUI Xin1,CAI Lei1(1.Key Laboratory of Non-wood Forest Products of State Forestry Administration,Central South University of Forestry and Technology,Changsha,Hunan 410004,China,2.Hunan Academy of Forestry Sciences,China) |
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| Abstract: | By using Camellia oleifera′s pollen as the material,the influence of 8 nutrient elements on germination percentage was studied by tissue culture in vitro,The result was as follows: the pollen germination was inhibited by CaCl2,Na2Mo4,ZnSO4 and KCl,especially by ZnSO4,while it was enhanced by CO(NH2)2,KH2PO4,H3BO3 and MgCl2,the optimum concentrations which raised pollen germination percentage were 0.1-0.15 g·L-1,0.1 g·L-1,0.05 g·L-1,0.05-0.10 g·L-1 respectively.The combination of 0.15 g·L-1 CO(NH2)2+0.15 g·L-1 H3BO3+0.05 g·L-1 MgCl2+0.05 g·L-1 KH2PO4 gave the best result,where the pollen germination percentage was 90.51%,which was 46.79% higher than CK. |
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| Keywords: | Camellia oleifera nutrient elements pollen germination percentage |
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