| 辣椒胞质雄性不育系(CMS)子叶培养植株再生(英文) |
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| 引用本文： | 邓明华,文锦芬,邹学校. 辣椒胞质雄性不育系(CMS)子叶培养植株再生(英文)[J]. （《农业科学与技术》）编辑部, 2009, 0(1) |
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| 作者姓名： | 邓明华 文锦芬 邹学校 |
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| 作者单位： | 中南大学隆平分院;云南农业大学园林园艺学院;昆明理工大学现代农业工程学院; |
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| 基金项目： | Supported by "863" High Tech Project of China (2001AA241121-10) ;;Natural Science Foundation of Yunnan Province (2005C0023Q)~~ |
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| 摘 要： | An in vitro shoot regeneration procedure was developed in pepper (Capsicum annuum L.) cytoplasmic male sterility (CMS) lines 9704A and 8214A using cotyledon as explant. The callus and bud cluster derived from cotyledon tissue explants were proliferated on Murashige and Skoog (MS) medium supplemented with different combinations of 6-benzladenine (6-BA), indole-3-acetic acid (IAA), gibberellic acid (GA3) and silver nitrate (AgNO3). From the formula of MS appended with 5.0 mg/L 6-BA, 1.0 mg/L IAA and 5.0 mg/L AgNO3, for the explants callus and bud cluster, the maximum differentiation rates (respectively 100.0% and 58.3%) and average number of adventitious bud from each explant (respectively 18.8 and 13.2) were obtained. The optimum medium combination for the elongation of adventitious bud was determined to be: MS+ 3.0 mg/L 6-BA + 1.0 mg/L IAA+ 5.0 mg/L AgNO3+ 2.0 mg/L GA3, from which the elongation rates of buds from callus and bud cluster were both 100%, and the average number of per explant adventitious bud number reached 6.3 and 5.8, respectively. And all the elongated shoots were successfully rooted on half-strength MS medium supplemented with 0.3-0.5 mg/L IAA.
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| 关 键 词： | 生根培养基 不定芽分化 细胞分裂素 基本培养基 子叶外植体 不同浓度 硝酸银 辣椒 愈伤组织 胞质雄性不育系 |
In vitro Plant Regeneration of Pepper Cytoplasmic Male Sterility (CMS) Lines via Cotyledon Culture |
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| DENG Ming-hua,,WEN Jin-fen,ZOU Xue-xiao. Branch of Longping,Central South University,Changsha ,. College of L,scape , Horticulture,Yunnan Agricultural University,Kunming ,. Faculty of Modern Agricultural Engineering,Kunming University of Science , Technology,Kunming. In vitro Plant Regeneration of Pepper Cytoplasmic Male Sterility (CMS) Lines via Cotyledon Culture[J]. 农业科学与技术(英文版)Agricultural Science ＆ Technology, 2009, 0(1) |
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| Authors: | DENG Ming-hua WEN Jin-fen ZOU Xue-xiao. Branch of Longping Central South University Changsha . College of L scape Horticulture Yunnan Agricultural University Kunming . Faculty of Modern Agricultural Engineering Kunming University of Science Technology Kunming |
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| Affiliation: | DENG Ming-hua1,2,WEN Jin-fen3,ZOU Xue-xiao11. Branch of Longping,Central South University,Changsha 410125,2. College of L,scape , Horticulture,Yunnan Agricultural University,Kunming 650201,3. Faculty of Modern Agricultural Engineering,Kunming University of Science , Technology,Kunming 650224 |
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| Abstract: | An in vitro shoot regeneration procedure was developed in pepper (Capsicum annuum L.) cytoplasmic male sterility (CMS) lines 9704A and 8214A using cotyledon as explant. The callus and bud cluster derived from cotyledon tissue explants were proliferated on Murashige and Skoog (MS) medium supplemented with different combinations of 6-benzladenine (6-BA), indole-3-acetic acid (IAA), gibberellic acid (GA3) and silver nitrate (AgNO3). From the formula of MS appended with 5.0 mg/L 6-BA, 1.0 mg/L IAA and 5.0 mg/L ... |
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| Keywords: | Capsicum annuum L. CMS line Cotyledon culture Plant regeneration |
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