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Identification of <Emphasis Type="Italic">Globodera rostochiensis</Emphasis> and <Emphasis Type="Italic">G. pallida</Emphasis> in the Ukraine by PCR
Authors:L?A?Pylypenko  T?Uehara  M?S?Phillips  D?D?Sigareva  Email author" target="_blank">V?C?BlokEmail author
Institution:(1) Institute of Plant Protection, Nematology Lab, Ukrainian Academy of Agrarian Sciences, 33 Vasilkovsky Str., Kyiv-22, 03022, Ukraine;(2) Laboratory of Plant Nematology, National Agricultural Research Centre for Hokkaido Region, 1 Hitsujigaoka, Toyohira-ku, Sapporo 062-8555, Japan;(3) Plant Pathogen Interactions Program, Scottish Crop Research Institute, Invergowrie, Dundee, Scotland, DD2 5DA, UK
Abstract:Multiplex polymerase chain reaction was used to identify the potato cyst nematodes in soil samples from the Ukraine. The results show the occurrence of Globodera pallida in the Uzhhorod region (Zakarpatsrsquoka oblastrsquo), where only G. rostochiensis had been previously reported. In the mixed potato cyst nematode (PCN) populations, G. pallida was less prevalent (2–5%) than G. rostochiensis (95–98%). A phylogenetic analysis based on ribosomal DNA internal transcribed spacer sequences showed that the Ukrainian population of G. pallida had >99% sequence identity with other G. pallida pa2/3 isolates from Europe. This study has demonstrated that polymerase chain reaction-mediated amplification of specific regions of the potato cyst nematode genome is not only highly effective as a species diagnostic tool but is also a sensitive method which can be used for taxonomic purposes with cyst collections which vary in age.
Keywords:diagnostics  internal transcribed spacer  phylogenetics  potato cyst nematodes
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