利用卡那霉素筛选野罂粟转基因植株的技术研究

待供试转基因和非转基因野罂粟植株长至5～7片叶时,用微型喷雾器将不同浓度的卡那霉素溶液均匀喷施于叶片上,结果显示浓度为6.5g/L的处理表现效果最好。对于大田筛选转基因野罂粟,在苗期对叶片喷施6.5g/L卡那霉素溶液喷雾,并对产生黄斑的野罂粟植株进行剔除后,拔节期用7.5g/L卡那霉素溶液连续5d涂抹后,对产生黄斑的植株进行再次剔除,最终得到30株未见黄斑的野罂粟植株,经PCR进行检测后,最终得到8株野罂粟转基因植株。

Engineering research of the wild opium poppy transgene plant by kanamycin mildew

Used different kanamycin density spurt executes on the opium poppy leaf blade with the miniature sprayer while the transgenic and the non-transgenic wild opium poppy plant growed 5 ～7 piece of leaves.The results demonstrated that the treatment of 6.5 g/L performanced was better. Wild opium poppy in field with 5～7 piece of leaves carried on the atomization, by using 6.5 g/L the kanamycin densities and yellow spot's adult plant carried on the rejection;in jointing stage carried on 5 d with 7.5 g/L kanamycin to smudge continuously, after the pair process yellow spot carried on rejects once more, 30 wild opium poppy plant without yellow spot were obtained, after PCR method examination 8 wild opium poppy transgene plant were obtained.