两花生品种对镉胁迫的生理响应及其差异

根据本实验室前期的水培实验,利用盆栽培养,研究了外加土壤Cd处理浓度为12 mg kg-1时花生植株的生理毒害反应及其品种间差异。以两个不同的花生品种花育22和花育20为实验材料进行对比研究,分别测定三个不同时期花生叶片叶绿素含量和细胞膜透性、根系和叶片丙二醛含量、抗氧化酶体系活力及花生产量。结果表明:在外加土壤Cd处理浓度为12mg kg-1时,叶片叶绿素含量和抗氧化酶体系活力随着处理时间的增加而降低,Cd处理组相对于对照组都有所下降,且花育20下降幅度要远大于花育22,尤其在成熟期,镉处理组中花育20的SOD、POD和CAT活力显著低于对照组;而细胞膜透性和丙二醛含量则是随着处理时间的增加而增加,且Cd处理组相对于对照组都有所增加,花育20增加幅度要大于花育22。产量测定结果显示,花育22下降13.14%,花育20下降26.98%。结合各指标的变化情况分析可知,花生对Cd污染存在着较大的品种间差异;其中过氧化物岐化酶(SOD)、丙二醛(MDA)和叶绿素三个指标变化幅度较大,为初步筛选选育抗Cd花生品种的参考指标和花生Cd污染的诊断指标提供依据。

Physiological Responses of Different Peanut Varieties to Cadmium Stress

According to early hydroponic experiment in our laboratory,pot cultivation experiment was conducted to investigate the physiological responses of two different peanut varieties(huayu 22 and huayu 20) on the plus soil Cd concentration of 12 mg kg.With this two peanut varieties as test materials,the chlorophyll content of leaves,malondialdehyde content and antioxidant enzyme activities of roots and leaves,cell membrane permeability and products were taking as test parameters.The results showed that when the plus soil Cd concentration was 12 mg kg,the chlorophyll content of leaves and antioxidant enzyme activities were reduced with processing time increase.The Cd treatment group had declined compared with the control group and the decline of huayu 20 was much larger than huayu 22.Especially in the mature period,the SOD,POD and CAT activity of huayu 20 in cadmium treatment group were significantly lower than control group.But the cell membrane permeability and malondialdehyde content were increased with processing time increase.The Cd treatment group had increased compared with the control group and the increase of huayu 20 was much larger than huayu 22.Determination of yield results showed that "huayu 22" and "huayu 20" were shrink by about 13.14 and 26.98 percent respectively.Combining with the analysis above,the Cd pollution had large difference between species of peanuts.Meanwhile SOD,MDA and chlorophyll content changed in a large extent which could as diagnosis index of cadmium pollution and reference index of cadmium varieties resistant.