| 罗田甜柿幼胚培养条件的研究 |
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| 引用本文： | 谷晓峰,唐仙英,罗正荣. 罗田甜柿幼胚培养条件的研究[J]. 果树学报, 2001, 18(2): 80-83 |
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| 作者姓名： | 谷晓峰 唐仙英 罗正荣 |
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| 作者单位： | 华中农业大学园艺系, |
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| 基金项目： | 国家自然科学基金，教育部优秀青年教师资助计划，高等学校博士学科专项科研基金资助项目 |
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| 摘 要： | 利用 5因素 4水平的正交试验对‘罗田甜柿’幼胚培养条件进行了探讨 ,结果表明 :(1)培养基以MS(1/ 2N)最适 ;(2 )蔗糖浓度以 7%为宜 ;(3)抗褐化剂以活性炭效果最好 ;(4 )最佳激素配比是IBA 0 .3mg/L +Zeatin0 .44mg/L ;(5 )激素种类生长素类以IAA为好 ,细胞分裂素类以Zeatin效果较好 ;(6 )MS(1/ 2N) +IAA 1mg/L +暗处理 4d生根率最高
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| 关 键 词： | 胚抢救 罗田甜柿 |
| 文章编号： | 1009-9980-(2001)-02-080-04 |
| 修稿时间： | 2000-03-13 |
A Study on Culture Conditions of Immature Embryo in Luotiantianshi Persimmon Variety |
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| GU Xiaofeng,Tang Xianying,Luo Zhengrong. A Study on Culture Conditions of Immature Embryo in Luotiantianshi Persimmon Variety[J]. Journal of Fruit Science, 2001, 18(2): 80-83 |
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| Authors: | GU Xiaofeng Tang Xianying Luo Zhengrong |
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| Abstract: | The factors influencing immature embryo culture in Luotiantianshi persimmon variety ( Diospyros kaki f.) were studied by Orthogonal design in 5 levels and 4 planes. The results showed that: (1) the optimum medium was MS(1/2N); (2) the optimum sucrose concentration was 7%; (3) activated carbon was the best for browning control; (4) the optimum IBA and Zeatin concentration was 0.3 mg/L and 0.44 mg/L ,respectively; (5) the optimum kind of auxin and cytokinin was IAA and Zeatin; (6) a higher frequency of rooting occurred on half strengthen MS(1/2N) medium supplemented with 1 mg/L IAA and dark treatment for 4 days. |
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| Keywords: | Embryo rescue Luotiantianshi persimmon |
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