抗生素对不同基因型矮牵牛分化及再生的影响

以矮牵牛4个品种美声晨光、黄金肉红、珊瑚红、黄金紫罗兰星条为试材，分别以卡那霉素Kan和头孢霉素Cef为选择抗生素和抑菌剂，研究不同浓度抗生素对叶片不定芽再生和试管苗生根的影响，分别筛选出适宜的抗生素浓度。结果表明：30mg/L Kan可以抑制叶片不定芽再生，50mg/L Kan条件下，无不定芽再生。低浓度Kan即可抑制试管苗不定根再生，30mg/L Kan条件下试管苗不再生根。550mg/L Cef条件下，叶片不定芽再生受到抑制，而试管苗生根的适宜Cef浓度是400mg/L Cef。

Effects of Antibiotic on Differentiation and Regeneration of Different Petunia hybride Genotypes

The effect of Kanamycin (Kan) and Cefotaxime (Cef) with different concentrations on adventitious bud differentiation from leaflets of Petunia hybride and rooting of the tube plantlets was studied by taking 4 cultivars, namely ‘Meishengchenguang’, ‘Huangjinrouhong’,‘Shanhuhong’ and ‘Huangjinziluolanxingtiao’ as experimental materials. The appropriate antibiotic concentrations were screened. The results showed that 30mg/L Kan could restrain adventitious bud differentiation, and no adventitious buds occurred when the concentration of Kan was 50mg/L.Similarly, low concentration of Kan restrained the rooting of tube plantlets, while no adventitious roots took place when Kan concentration was 30mg/L. The occurrence of adventitious buds were limited under the condition of 550mg/L Cef, and 400mg/L Cef was the suitable concentration for adventitious bud differentiation.