蝴蝶兰高效再生体系与遗传转化体系的建立

为建立蝴蝶兰(Phalaenopsis amabilis)再生体系和遗传转化体系,对蝴蝶兰进行人工授粉,成熟后将胚播种于诱导培养基上诱导原球茎或不定芽。探讨外源调节剂的种类及浓度,并筛选卡那霉素浓度以及除菌剂的种类及浓度。结果表明:当6-BA浓度为5 mg·L~(~(-1)),NAA浓度为1 mg·L~(-1)时,原球茎的诱导效率最高。当6-BA浓度为1.5mg·L~(-1),同时添加0.5g·L~(-1)活性炭和40mL·L~(-1)椰汁时,壮苗效果最佳。卡那霉素的筛选浓度为4mg·L~(-1),除菌剂的种类为阿莫西林克拉维酸钾(7∶1),除菌浓度为100mg·L~(-1)。

Establishment of Efficient Regeneration System and Genetic Transformation System of Phalaenopsis

In order to establish the regeneration system and genetic transformation system of Phalaenopsis amabilis,Phalaenopsis amabilis was used for artificial pollination,after maturation,the embryos were seeded on the induction medium to induce protocorm or adventitious buds.The type and concentration of exogenous regulators were explored,and the concentration of kanamycin and the type and concentration of the fungicide were screened.The results showed that when the concentration of 6-BA was 5 mg·L.-1 and NAA was 1 mg·L.-1,the induction efficiency of protocorm was the highest.When the concentration of 6-BA 1.5 mg·L.-1,while adding 0.5 g·L.-1 activated carbon and 40 mL·L.-1 coconut,the best effect of strong seedlings.The results showed that the concentration of kanamycin was 4 mg·L.-1,the type of fungicide was amoxicillin clavulanate (7:1),and the concentration was 100 mg·L.-1.