绿萝叶斑病病原菌鉴定及外源水杨酸对其影响

为探明绿萝患病叶片的病原菌,采用形态学鉴定及分子生物学方法鉴定其病原菌来源,进一步探究外源物质对于此病原菌的影响。并采用施加外源水杨酸观测病原菌菌落生长情况、抑菌率及病原孢子萌发情况。结果表明:绿萝病害为叶斑病,此病原菌为枝孢杆菌;在不同浓度外源水杨酸配置的PDA培养基菌落增长速度观察中,低浓度0.1mmol·L~(-1) SA会促使菌落增长,而随着水杨酸浓度增加,菌落生长速度降低,2.0~10.0mmol·L~(-1)的测试组始终对病原菌有极高的抑制作用,并没有菌丝体产生。病原孢子数量在不同浓度的水杨酸影响下有着明显的变化。低浓度水杨酸对叶斑病病原孢子生长有促进作用,而0.3、0.5、1.0mmol·L~(-1)水杨酸对病原菌孢子萌发有着较好的抑制性。

Pathogen Identification for Epipremnum aureum Leaf Spot and the Effect of Exogenous Salicylic Acid

Methods of morphological and molecular biological identification were adopted to ascertain the pathogens of Epipremnum aureum leaf spot.To go a step further on the effect of exogenous factors on pathogens,exogenous salicylic acid was used in the study to observe the growing conditions of pathogen colony,bacteriostatic rate and the germination situation of the pathogen spores.It was found that the Epipremnum aureum had been infected with leaf spot,which fell into Bacillus cladospore.For bacterial colony cultured by PDA medium produced with exogenous salicylic acid of different concentrations,it was noticed that the low concentration of salicylic acid for 0.1 mmol·L-1would facilitate the growth of bacterial colony.With the growth of salicylic acid concentration,the growth of bacterial colony decelerated.The testing group,experimented with the concentration of 2.0~10.0 mmol·L-1,exerted constantly high inhibiting effects on pathogens,without mycelium generated.With the change in salicylic acid concentration,the number of pathogenic spores varied significantly.Low salicylic acid concentration would facilitate the pathogenic spore growth of leaf spot.However,the salicylic acid concentration for 0.3~1.0 mmol·L-1had a negative effect on the reproduction of pathogenic spores.It exerted a significantly inhibiting effect on the growth of pathogenic spores.