人参茎叶皂苷对醛糖还原酶的抑制作用

以五年生人参茎叶为材料,提取总皂苷,用30%、50%、80%乙醇洗脱人参茎叶总皂苷获取不同种类和含量人参皂苷,测定不同浓度乙醇洗脱人参茎叶皂苷对醛糖还原酶(AR)抑制作用。结果表明:不同浓度乙醇洗脱人参茎叶皂对AR都有一定的抑制作用,其中对AR抑制作用最强的是80%乙醇洗脱人参茎叶皂苷对AR抑制作用超过阳性对照依帕司他(EPS)(P0.01),30%乙醇洗脱人参茎叶皂苷和50%乙醇洗脱人参茎叶皂苷对AR抑制作用有一定的抑制,但没有超过EPS。醛糖还原酶抑制剂对糖尿病并发症,尤其是对糖尿病微血管并发症有很好的治疗效果。综上所述,人参茎叶提取总皂苷在糖尿病微血管并发症治疗方面有一定的应用价值。

Study on Inhibitory Effect of Saponin of Ginseng Stems and Leavea on Aldose Reductase

The ginseng stems and leaves that had 5 years old as material,which were extracted total ginsenosides,which different types and contents of ginsenoside elutedwith 30%,50% and 80% ethanol,and different concentrations of ethanol elution ginseng stems and leaves ginsenoside on aldose reductase (AR) inhibition were determinated.The results showed that the elution of 80% ethanol had the strongest inhibitory effects on aldos reductase (AR),which was significantly stronger than that of the positive control of EPS(P<0.01);the elutions of 30% and 50% ethanol both had certain inhibitory effects on AR,while that was not better than that of EPS.Aldose reductase inhibitor especially had a good therapeutic effect on diabetic microvascular complications.Above all,the total ginsenoside of ginseng stems and leaves had certain application value in the treatment of diabetic microvascular complications.