Immunoperoxidase plaque staining for the detection of pseudorabies virus. |
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Authors: | A Afshar and G C Dulac |
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Abstract: | A quantitative indirect immunoperoxidase plaque staining method was developed for the detection of pseudorabies virus infection in a pig kidney cell line (PK-15). The method is rapid and specific and foci of infection, represented by stained plaques, are easily counted by the unaided eye. Possible modification of this technique in a plaque reduction assay for the detection of antipseudorabies virus antibody is also discussed. |
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