| HPLC法测定杜仲中绿原酸和芦丁 |
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| 引用本文： | 李艳艳,周光明,辛丹敏,梁丽芳. HPLC法测定杜仲中绿原酸和芦丁[J]. 西南大学学报(自然科学版), 2010, 32(1) |
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| 作者姓名： | 李艳艳 周光明 辛丹敏 梁丽芳 |
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| 作者单位： | 发光与实时分析教育部重点实验室;西南大学化学化工学院; |
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| 基金项目： | 发光与实时分析重庆市重点实验室(西南大学)(CSTC,2006CA8006); 西南大学校青年基金(SWNUQ,2005008); 重庆市自然科学基金资助项目(CSTC,2007BB5370) |
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| 摘 要： | 采用phenomenex C18色谱柱(150 mm×4.6 mm,5μm),以甲醇-冰醋酸(pH=3.10)(45∶55)为流动相洗脱,体积流量为0.8 mL/min,紫外检测波长为327 nm,采用外标法测定不同产地杜仲中绿原酸和芦丁的含量.建立了HPLC法测定杜仲中绿原酸和芦丁含量,并对不同产地杜仲中绿原酸和芦丁含量进行测定.结果表明,绿原酸和芦丁分别在0.127 5～24.000 0μg(r=0.999 6),0.125 0～25.000 0μg(r=0.999 6)线性关系良好,平均回收率分别为98.73%和97.26%,相对标准偏差为1.85%和1.32%.
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| 关 键 词： | 高效液相色谱 杜仲 绿原酸 芦丁 |
Determination of Chlorogenic Acid and Rutin in Eucommia ulmoides Oliv by HPLC |
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| LI Yan-yan,ZHOU Guang-ming,XIN Dan-min,LIANG Li-fang Key Laboratory on Luminescence , Real-Time Analysis,Ministry of Education,Chongqing ,China,School of Chemistry , Chemical Engineering,Southwest University. Determination of Chlorogenic Acid and Rutin in Eucommia ulmoides Oliv by HPLC[J]. Journal of southwest university (Natural science edition), 2010, 32(1) |
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| Authors: | LI Yan-yan ZHOU Guang-ming XIN Dan-min LIANG Li-fang Key Laboratory on Luminescence Real-Time Analysis Ministry of Education Chongqing China School of Chemistry Chemical Engineering Southwest University |
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| Affiliation: | LI Yan-yan,ZHOU Guang-ming,XIN Dan-min,LIANG Li-fang Key Laboratory on Luminescence , Real-Time Analysis,Ministry of Education,Chongqing 400715,China,School of Chemistry , Chemical Engineering,Southwest University |
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| Abstract: | |
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| Keywords: | HPLC Eucommia ulmoides Oliv chlorogenic acid rutin |
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