| 肠毒素型大肠杆菌F18菌毛对产蛋鸡免疫的原性 |
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| 引用本文： | 由昭红,姜中其.肠毒素型大肠杆菌F18菌毛对产蛋鸡免疫的原性[J].福建农林大学学报(自然科学版),2009,38(6). |
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| 作者姓名： | 由昭红 姜中其 |
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| 作者单位： | 1. 枣庄学院旅游与资源环境系,山东,枣庄,277160
2. 浙江大学动物科学学院,浙江,杭州,310029 |
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| 基金项目： | 浙江省科技厅资助项目 |
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| 摘 要： | 提取猪源肠毒素型大肠杆菌(ETEC)标准株和临床分离株F18菌毛,并制备弗氏佐剂苗,分别免疫产蛋母鸡后用间接ELISA法定期检测母鸡卵黄抗体和血清抗体效价.结果表明:无论标准株还是分离株,F18菌毛对产蛋鸡均具有良好的免疫原性,卵黄抗体效价最高可达1∶25600,血清抗体效价高于卵黄抗体效价,最高可达1∶51200.
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| 关 键 词： | F18菌毛 产肠毒素型大肠杆菌 免疫原性 |
Immune effect of entertoxigenic Escherichia coli F18 fimbriae vaccines on laying hens |
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| YOU Zhao-hong,JIANG Zhong-qi.Immune effect of entertoxigenic Escherichia coli F18 fimbriae vaccines on laying hens[J].Journal of Fujian Agricultural and Forestry University,2009,38(6). |
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| Authors: | YOU Zhao-hong JIANG Zhong-qi |
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| Institution: | YOU Zhao-hong1,JIANG Zhong-qi2(1.Department of Tourism and Resoure Environment,Zaozhuang University,Zaozhuang,Shandong 277160,China,2.College of Animal Science,Zhejiang University,Hangzhou,Zhejiang 310029,China) |
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| Abstract: | F18 fimbriae of enterotoxigenic Escherichia coli (ETEC) standard strain and local isolates were respectively extracted with thermoextract method. Vaccines were made of each fimbriae and Freund′s adjuvant and two groups of laying hens were immunized respectively. And then, the antibodies were detected by indirect enzyme-linked immunosorbent assay (ELISA). The results indicated that two fimbriae could induce significant immune responses, the serum antibody titer reached 1:51200 and the egg yolk antibody titer reached 1:25600. |
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| Keywords: | F18 fimbriae enterotoxigenic Escherichia coli immunogenicity |
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