| 辣椒抗黄瓜花叶病毒QTL分析 |
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| 引用本文： | 赵娟,王立浩,毛胜利,张正海,云兴福,张宝玺. 辣椒抗黄瓜花叶病毒QTL分析[J]. 中国蔬菜, 2011, 1(18): 30-35 |
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| 作者姓名： | 赵娟 王立浩 毛胜利 张正海 云兴福 张宝玺 |
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| 作者单位： | 1.中国农业科学院蔬菜花卉研究所，北京 100081,2内蒙古农业大学农学院，内蒙古呼和浩特010019 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项(201109) |
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| 摘 要： | 利用辣椒抗黄瓜花叶病毒材料perennial与园艺性状优良的感病材料茄门，及其F2群体146个单株、F3 146个株系为试材，采用SRAP和SSR分子标记技术，利用JoinMap 3.0软件构建了包含76个标记、13个连锁群，覆盖长度为830.4 cM的辣椒分子遗传图谱。对F3株系（4 380个单株）进行人工接种鉴定黄瓜花叶病毒，其病情指数呈正态分布。结合分子遗传图谱的结果，利用MapQTL 4.0软件进行分析，将抗病基因的QTL定位在第1、4、7连锁群上，抗性贡献率分别为12.7%、38.8%、11.0%。
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| 关 键 词： | 辣椒 抗CMV SRAP SSR QTL |
QTL Analysis of Cucumber Mosaic Virus Resistance in Pepper (Capsicum annuum L.) |
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| ZHAO Juan , WANG Li-hao , MAO Sheng-li , ZHANG Zheng-hai , YUN Xin-fu , ZHANG Bao-xi. QTL Analysis of Cucumber Mosaic Virus Resistance in Pepper (Capsicum annuum L.)[J]. China Vegetables, 2011, 1(18): 30-35 |
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| Authors: | ZHAO Juan WANG Li-hao MAO Sheng-li ZHANG Zheng-hai YUN Xin-fu ZHANG Bao-xi |
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| Affiliation: | 1.Institution of Vegetable and Flower，Chinese Academy of Agricultural Science，Beijing 100081，China|2Inner Mongolia Agricultural University，Huhhot 010019，Inner Mongolia，China |
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| Abstract: | In this paper,a F2 population including 146 individuals were used for research,which were derived from CMV resistant pepper(Capsicumm annuum L.) inbred line'perennial'and sensitive inbred line'Qiemen'with good horticultural traits.Based on screening the SRAP and SSR primers and analyzing genotypes of the F2 population,a genetic linkage map of pepper was constructed.F3 population(4 380 individuals)which derived from F2 146 selfed individuals were used for inoculate CMV.Then 3 main QTLs were located in linkag... |
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| Keywords: | Pepper CMV resistance SRAP SSR QTL |
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