| 兴安百里香离体快繁技术研究 |
| |
| 引用本文： | 王新颖.兴安百里香离体快繁技术研究[J].中国城市林业,2014(6):23-25. |
| |
| 作者姓名： | 王新颖 |
| |
| 作者单位： | 沈阳市园林科学研究院,沈阳110016 |
| |
| 摘 要： | 为提高兴安百里香繁殖速度,研究了兴安百里香的离体快繁技术。研究结果表明:以带腋芽茎段为外植体,1g·LHgCl2消毒7 min为宜;适宜的诱导愈伤组织培养基为MS+6-BA0.25 mg·L-1+IAA1.0 mg·L-1,适宜的愈伤组织分化培养基为MS+6-BA0.5 mg·L-1+IAA0.5 mg·L-1,适宜的继代增殖培养基为MS+IBA0.5 mg·L-1,适宜的最佳生根培养基为1/2MS+IBA0.5 mg·L-1。
|
| 关 键 词： | 兴安百里香 离体繁殖 培养基 |
Research on Fast in vitro Propagation Technology for Thymus dahuricus |
| |
| Wang Xinying.Research on Fast in vitro Propagation Technology for Thymus dahuricus[J].Journal of Chinese Urban Forestry,2014(6):23-25. |
| |
| Authors: | Wang Xinying |
| |
| Institution: | Wang Xinying ( Shenyang Academy of Landscaping, Shenyang 110016, China) |
| |
| Abstract: | In order to improve the reproduction rate of Thymus dahuricus,the fast in vitro propagationtechnology was studied. The results indicated that the stem segment with buds was the best explant,and the optimum sterilization time was 7 min in1g·L HgCl2; MS ＋ 6- BA0. 25 mg·L^- 1＋ IAA1. 0 mg·L^- 1was the optimum medium for primary culture; the most suitable medium for callus differentiation was MS ＋ 6- BA0. 5 mg·L^- 1＋ IAA0. 5 mg·L^- 1; the most suitable medium for proliferation was MS ＋ IBA0. 5 mg·L^- 1; and 1 /2MS ＋ IBA0. 5 mg·L^- 1was the best medium for root induction. |
| |
| Keywords: | Thymus dahuricus in vitro propagation medium |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
