| 橡胶树胶孢炭疽病菌致病相关基因CgATG8的功能分析 |
| |
| 引用本文： | 李超萍,林春花,翟李刚,时 涛,黄贵修. 橡胶树胶孢炭疽病菌致病相关基因CgATG8的功能分析[J]. 热带作物学报, 2013, 34(11): 2172-2178 |
| |
| 作者姓名： | 李超萍 林春花 翟李刚 时 涛 黄贵修 |
| |
| 作者单位： | 中国热带农业科学院环境与植物保护研究所/农业部热带作物有害生物综合治理重点实验室/海南省热带农业有害生物监测与控制重点实验室;中国热带农业科学院环境与植物保护研究所/农业部热带作物有害生物综合治理重点实验室/海南省热带农业有害生物监测与控制重点实验室;中国热带农业科学院环境与植物保护研究所/农业部热带作物有害生物综合治理重点实验室/海南省热带农业有害生物监测与控制重点实验室;中国热带农业科学院环境与植物保护研究所/农业部热带作物有害生物综合治理重点实验室/海南省热带农业有害生物监测与控制重点实验室;中国热带农业科学院环境与植物保护研究所/农业部热带作物有害生物综合治理重点实验室/海南省热带农业有害生物监测与控制重点实验室 |
| |
| 基金项目： | 国家天然橡胶产业技术体系建设项目（No. CARS-34-GW8）；中央级公益性科研院所基本科研业务费专项（No. 1630042013016）；国家自然科学基金项目（No. 31201468）。 |
| |
| 摘 要： | ATG8是病原真菌中细胞自噬过程中的重要基因，并参与致病过程。基于橡胶树胶孢炭疽菌HBCg01全基因组数据库，采用PCR和RT-PCR扩增得到橡胶树胶孢炭疽病菌的CgATG8基因并进行序列分析。采用Split-Marker Recombination法获得该基因的敲除转化子。表型分析结果表明，缺失该基因后，橡胶树胶孢炭疽病菌的致病力丧失，产孢能力、孢子萌发率、附着胞形成率和膨压均降低，而且孢子萌发和附着胞的形成延迟。
|
| 关 键 词： | 橡胶树；胶孢炭疽病菌；CgATG8 |
Functional Analysis of Pathogenic Related Gene CgATG8 from Colletotrichum gloeosporioides |
| |
| LI Chaoping,LIN Chunhu,ZHAI Ligang,SHI Tao and HUANG Guixiu. Functional Analysis of Pathogenic Related Gene CgATG8 from Colletotrichum gloeosporioides[J]. Chinese Journal of Tropical Crops, 2013, 34(11): 2172-2178 |
| |
| Authors: | LI Chaoping LIN Chunhu ZHAI Ligang SHI Tao HUANG Guixiu |
| |
| Affiliation: | Environment and Plant Protection Institute, CATAS, Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture, Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests;Environment and Plant Protection Institute, CATAS, Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture, Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests;Environment and Plant Protection Institute, CATAS, Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture, Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests;Environment and Plant Protection Institute, CATAS, Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture, Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests;Environment and Plant Protection Institute, CATAS, Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture, Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests |
| |
| Abstract: | ATG8 is an important gene involved in the cell autophagy process, which is involved in the fungal infection process. Based on the rubber Colletotrichum gloeosporioides strain HBCg01 genome database, CgATG8 of C. gloeosporioides was cloned and sequenced. The deletion mutant of CgATG8 was obtained by Split-Marker Recombination method. The morphology and virulence tests showed that the mutant reduced the ability of sporulation, conidia germination, appressorium formation and turgor. The conidia germination and appressorium formation were delayed too. |
| |
| Keywords: | |
|
| 点击此处可从《热带作物学报》浏览原始摘要信息 |
|
点击此处可从《热带作物学报》下载全文 |
|
