白斑综合征病毒感染克氏原螯虾后的PCR检测及组织病理学研究

采用投喂感染白斑综合征病毒(White Spot Syndrome Virus,WSSV)对虾肌肉的方式,对养殖克氏原螯虾(Procambarus clarkii)进行人工感染,以确定WSSV对养殖克氏原螯虾的易感性。结果发现,投喂病虾感染组螯虾的死亡率达到90%,而对照组未出现死亡。采用PCR对试验组螯虾的肌肉进行WSSV检测,发现投喂感染组的阳性检出率为100%,对照组的阳性检出率均为0。PCR检测结果发现,濒死螯虾的肝胰腺、中肠、肌肉、鳃、性腺、心脏六种组织的PCR结果均为WSSV阳性,而对照组的各组织检测结果均为阴性。组织切片的光镜观察也证实,濒死螯虾的肝胰腺、中肠、肌肉、鳃、性腺、心脏及血淋巴等组织均发生了不同程度的病变。

PCR detection of White Spot Syndrome Virus infected Procambarus clarkii and histopathological study

Experimental infection of crayfish,Procambarus clarkii,were conducted by feeding white spot syndrome virus(WSSV) infected shrimp,Fenneropenaeus chinensis to determine the susceptibility of crayfish to WSSV.The results showed that a cumulative mortality of 90% was observed in the infected crayfish,while no death in control group.Crayfish that dead in oral infection was positive for the presence of WSSV by PCR.PCR detection results also showed that six tissues including hepatopancreas,mid-intestine,muscle,gills,gonad and heart of dying crayfish were positive to WSSV,while those were negative in control group.Optical microscope observation also proved that hepatopancreas,mid-intestine,muscle,gills,gonad,heart and hemolymph of dying crayfish showed pathological variation to different degree.