禾谷缢管蚜、麦长管蚜乙酰胆碱酯酶基因片段的克隆与序列分析

采用RT—PCR技术，设计简并上、下游引物，从禾谷缢管蚜（Rhopalosiphum padi（Linnaeus））和麦长管蚜（Sitobion avenae （Fabricius））中均克隆出2种不同乙酰胆碱酯酶的cDNA片段，分别命名为Rp.acel、Rp.ace2和Sa.acel、Sa.ace2。同源性分析结果表明，Rp.acel、Sa.acel与棉蚜（Aphis gossypii）和桃蚜（Myzus persicae）的Ⅰ型乙酰胆碱酯酶（Acel）基因序列具有96％以上的相似性，Rp.ace2和Sa.ace2与棉蚜和桃蚜的Ⅱ型乙酰胆碱酯酶（Ace2）以及从麦二叉蚜（Schizaphis graminum）中克隆出的乙酰胆碱酯酶基因序列具有95％以上的相似性。说明禾谷缢管蚜和麦长管蚜体内都存在2种乙酰胆碱酯酶基因，而且预示蚜虫体内可能普遍存在2种不同的乙酰胆碱酯酶基因，这2种基因都具有相应的功能。

Cloning and sequence analysis of fragments of acetylcholinesterase genes from Rhopalosiphum padi ( Linnaeus) and Sitobion avenae (Fabricius)

Using degenerate primers and RT-PCR technique,two cDNA fragments of acetylcholinesterase genes were cloned from both Rhopalosiphum padi(Linnaeus)and Sitobion avenae(Fabricius)and were named as Rp.ace1,Rp.ace2,Sa.ace1 and Sa.ace2,respectively.They were 282,282,264 and 572 bp in length,and encodes 94,94,88 and 190 amino acids,respectively.Sequence analysis showed that Rp.ace1 and Sa.ace1 had more than 96% homology with the Ace1 from Aphis gossypii and Myzus persicae.Rp.ace2 and Sa.ace2 had more than 95% homology with the Ace2 from these two aphids and the Schizaphis graminum.Results implied that both Rhopalosiphum padi(Linnaeus)and Sitobion avenae(Fabricius)have two acetylcholinesterase genes,and the two genes may be popular in all aphids and both functional.