| 牛病毒性腹泻病病毒NS3表位串联蛋白表达及抗体间接ELISA方法的建立 |
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| 引用本文： | 贾莹,李岩,尹鑫,温凯,刘华,张文龙,王君伟. 牛病毒性腹泻病病毒NS3表位串联蛋白表达及抗体间接ELISA方法的建立[J]. 畜牧兽医学报, 2011, 42(8) |
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| 作者姓名： | 贾莹 李岩 尹鑫 温凯 刘华 张文龙 王君伟 |
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| 作者单位： | 东北农业大学动物医学学院,哈尔滨,150030 |
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| 基金项目： | 现代农业产业技术体系建设专项资金(NYCYTX-0303) |
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| 摘 要： | 为建立一种有效的牛病毒性腹泻病病毒(Bovine viral diarrhea virus,BVDV)抗体检测方法,作者将BVDVNS3蛋白2个抗原表位的编码核酸序列进行串联,构建重组表达载体pET-30a-EXnN,并在原核表达系统中表达了重组蛋白。选取包含12个表位肽的重组蛋白(r-BVDV-EX6N)作为包被抗原建立NS3蛋白抗体间接ELISA方法。该方法的特异性和稳定性良好,与2种商品化试剂盒的符合率分别为96.05%和78.95%。试验结果表明建立的ELISA方法可用于BVDV NS3蛋白抗体的检测。
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| 关 键 词： | BVDV NS3蛋白 表位串联 间接ELISA |
Development of Indirect ELISA of Bovine Viral Diarrhea Virus by Using Tandem Epitopes of NS3 |
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| JIA Ying,LI Yan,YIN Xin,WEN Kai,LIU Hua,ZHANG Wen-long,WANG Jun-wei. Development of Indirect ELISA of Bovine Viral Diarrhea Virus by Using Tandem Epitopes of NS3[J]. Chinese Journal of Animal and Veterinary Sciences, 2011, 42(8) |
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| Authors: | JIA Ying LI Yan YIN Xin WEN Kai LIU Hua ZHANG Wen-long WANG Jun-wei |
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| Affiliation: | JIA Ying,LI Yan,YIN Xin,WEN Kai,LIU Hua,ZHANG Wen-long,WANG Jun-wei (College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China) |
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| Abstract: | The present experiment was performed to establish an indirect ELISA for detection of antibodies against Bovine viral diarrhea virus(BVDV).Tandem epitopes of NS3 of BVDV was cloned into pET-30a and expressed in E.coli Rosetta.SDS-PAGE analysis revealed a band of protein correspondent with molecular weight of the target protein,and the result of western blot showed that the recombinant protein was recognized specifically by anti-BVDV positive serum.An indirect ELISA(r-BVDV-EX6N-ELISA)was developed using purif... |
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| Keywords: | Bovine viral diarrhea virus(BVDV) NS3 protein tandem epitopes indirect ELISA |
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