| 乳链菌肽发酵玉米浆—糖蜜培养基的优化制备 |
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| 引用本文： | 逯家辉,孙莹,姜丽艳,孟庆繁,李扬,滕利荣.乳链菌肽发酵玉米浆—糖蜜培养基的优化制备[J].农业机械学报,2009,40(7):158-162. |
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| 作者姓名： | 逯家辉 孙莹 姜丽艳 孟庆繁 李扬 滕利荣 |
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| 作者单位： | 吉林大学生命科学学院,长春,130012 |
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| 基金项目： | 吉林大学国家级生物实验教学示范中心设计创新实验基金一类资助项目 |
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| 摘 要： | 采用单因素试验方法对影响乳链菌肽效价的培养基组分进行了考察.结果表明,影响乳链菌肽效价的主要因素为糖蜜、玉米浆、K2HPO4、MgSO4.在此基础上,采用Box-Behnken设计及响应面分析法确定主要影响因子的最佳质量浓度.结果表明,当玉米浆质量浓度为233 g/L,糖蜜质量浓度为27 g/L,K2HPO4质量浓度为5.0 g/L ,MgSO4质量浓度为0.1 g/L时,乳链菌肽效价为2 491.8 IU/mL,与预测值2 511.1 IU/mL基本一致.
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| 关 键 词： | 乳链菌肽 玉米浆 糖蜜 Box-Behnken设计 响应面法 |
Optimization Corn Steep Liquor-Molasses Fermentation Medium for Nisin |
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| Lu Jiahui,Sun Ying,Jiang Liyan,Meng Qingfan,Li Yang,Teng Lirong.Optimization Corn Steep Liquor-Molasses Fermentation Medium for Nisin[J].Transactions of the Chinese Society of Agricultural Machinery,2009,40(7):158-162. |
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| Authors: | Lu Jiahui Sun Ying Jiang Liyan Meng Qingfan Li Yang Teng Lirong |
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| Institution: | College of Life Science;Jilin University;Changchun 130012;China |
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| Abstract: | This work reviewed fermentation medium culture of nisin by single factor experiment.The results indicate that nisin activity was mainly influenced of molasses,corn steep liquor,K2HPO4 and MgSO4.Box-Behnken design and response surface analysis methods were applied to confirm optimum concentration of mainly effect factor.The results show that the optimum medium culture was corn steep liquor 233 g/L,molasses 27 g/L,K2HPO4 5 g/L,MgSO4 0.1 g/L.Under those conditions,nisin activity is 2 491.8 IU/mL,which is in co... |
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| Keywords: | Nisin Corn steep liquor Molasses Box-Behnken design Response surface methodology |
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