外源一氧化氮对渗透胁迫下小麦幼苗叶片膜脂过氧化的影响

以永良4号小麦品种为试验材料,研究了不同浓度SNP(0.2、0.5、1.0 mmol/L)处理对渗透胁迫下(10%PEG-6000)小麦幼苗叶片膜脂过氧化的影响.结果表明:低浓度SNP(0.2 mmol/L)可以在处理后期(第2和第3天)显著地缓解渗透胁迫下小麦幼苗叶片的氧化损伤,并与其超氧化物歧化酶(SOD)、过氧化物酶(POD)活性以及渗透调节物质脯氨酸(Pro)含量的上升作用一致,与超氧阴离子(O2-.)的释放速率以及丙二醛(MDA)含量的下降一致.高浓度的SNP(1.0 mmol/L)则抑制这两种酶的活性和脯氨酸含量的增加,使O2-.产生速率和MDA含量相对升高.

Effects of exogenous nitric oxide on membrane lipid peroxidation in wheat leaves under osmotic stress

Effects of nitric oxide(NO)donor,sodium nitroprusside(SNP),with different concentrations on lipid peroxodation in wheat seedling leaves under 10 % PEG-6000 osmotic stress were investigated through 3 d of treatment.The results indicated that low concentration of SNP(0.2 mmol/L) could apparently alleviate the osmotic stress-induced lipid peroxidation during the late period of treatment(after 2 day treatment),which consistent with the up-regulation of enzyme activities,such as SOD,POD and the content of proline;consistent with the down-regulation of O-·2 production speed and the content of MDA.Meanwhile,high concentration of SNP(1.0 mmol/L) suppressed the activities of SOD and POD enzymes and content of proline,enhanced the O-·2 production speed and the content of MDA.