朱砂根组织培养防褐化探讨

探讨了朱砂根继代培养中抗氧化剂(维生素C、L-半胱氨酸、硫代硫酸钠)和吸附剂(活性炭、聚乙烯吡咯烷酮)对外植体褐化的影响。结果表明:培养基中添加活性炭3 g.L-1,继代周期控制在30 d之内,平均褐化率可控制在18.89%,效果最理想;培养基中添加维生素C 0.2 g.L-1也能有效控制褐化,但整体效果不如活性炭。

Pilot Study on Browning Control in Tissue Culture of Ardisia crenata Sims

The paper researched the prevention of browning in tissue culture of Ardisia crenata Sims with the effect of application of anti-browning agents,including 3 antioxidants(vitamin C,L-cysteine,Na2S2O3)and 2 absorbents(activated carbon,Polyvinyl Pyrrolidone).The results showed that,when 3 g·L-1 of activated carbon was added to medium and the subculture cycle was controlled in 30 days,the browning rate could effectively reduced to 18.89%,which was the best treatment in this study.The browning was also controlled by adding 0.2 g·L-1 of vitamin C to medium,however,the effect of browning control of vitamin C was not as good as the activated carbon's.