| 速眠新对大鼠原代肝细胞CYP3A1、CYP2E1的影响 |
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| 作者姓名： | 徐丽娜 杜金梁 马玉忠 |
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| 作者单位： | 河北农业大学动物科技学院 |
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| 基金项目： | 河北农业大学校科研和教改项目 |
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| 摘 要： | 胶原酶二步灌流法获取原代大鼠肝细胞,用不同浓度的速眠新和保定宁处理,Western blot法测定肝细胞中细胞色素酶P450 3A1(CYP3A1)和细胞色素酶P4502E1(CYP 2E1)的表达水平.结果表明:通过胶原酶灌流法,每只大鼠可获得2～4×108个肝细胞,成活率约为95%.用速眠新、保定宁处理后,肝细胞CYP3A1和CYP2E1的表达随速眠新、保定宁浓度的增加和处理时间的延长而呈升高的趋势.原代大鼠肝细胞可用于速眠新药物代谢分子机制的研究,为其临床上合理地应用提供了理论依据.
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| 关 键 词： | 速眠新 大鼠 原代肝细胞 |
Effects of SU-MIAN-XIN on CYP3A1 and CYP2E1 in Rat Primary Cultured Hepatocytes |
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| Authors: | Xu Lin Du Jinliang Ma Yuzhong |
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| Abstract: | Rat primary cultured hepatocytes were isolated by two-step collagenase digestion method and treated with gradient concentration of SU-MIAN-XIN and BAO-DING-NING, and the expression of cytochrome P450 3A1 (CYP3A1) and cytochrome P450 2E1 (CYP 2E1) were determined by Western blot. The results as follows: 2-4×108 hepatocytes per rat were obtained and the viability was about 95%. The expression of CYP3A1 and CYP 2E1 in rat hepatocytes increased gradually with treatment of SU-MIAN-XIN and BAO-DING-NING. Rat primary cultured hepatocytes can be used for molecular mechanism of SU-MIAN-XIN metabolism, it provides a foundation for its clinical application. |
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| Keywords: | CYP3A1:CYP2E1 |
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