铁在寒地早粳花药培养中的效应研究 |
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作者姓名: | 张兰民 黄晓群 潘国君 王瑞英 刘传雪 张淑华 关世武 冯雅舒 李大林 |
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作者单位: | 黑龙江省农业科学院水稻研究所,佳木斯154026 |
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摘 要: | 铁是水稻花培培养基的重要成分.通过对7份寒地早粳组合F1(或品系)花药在不同Fe2 含量培养基上离体培养,发现培养基中的Fe2 的含量对诱导率、绿苗分化率影响较大.结果表明在N6培养基中将Fc2 提高到32~40mg/kg诱导率最大,高于N6培养基(Fe2 含量为5.6mg/kg)1.4倍以上.在此以内随着Fe2 含量的增加诱导率增加,大于此含量诱导率降低,呈单峰曲线分布.分化培养基中Fe2 含量增加到40mg/kg分化率显著降低.但在32~40mg/kgFe2 培养基上诱导出的愈伤组织在MS培养基上的绿苗分化率差异明显,85.7%材料可使绿苗生产率提高到7.8%左右.因此适当提高诱导培养基中Fe2 含量可提高寒地早粳的花培效率.
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关 键 词: | 寒地早粳 花培 培养基 铁的效应 |
The iron effect study of early japonica anther culture in cold region |
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Authors: | Zhang Lanmin Huang Xiaoqun Pan Guojun Wang Ruiying Liu Chuanxue Zhang Shuhu Guan Shiwu Feng Yashu Li Dalin |
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Abstract: | Abstract: Iron is an important component in anther culture medium of rice. In this study, through vitro culturing anthers of 7 early japonica combinations F1 of cool region (lines) in medium of different Fe2+ content , we found that Fe2+ content have greater impact in the induction rate and the rate of green plant on differentiation. The results showed that Fe2+ will be increased to 32-40 mg/kg had largest induced rate in N6 medium, higher than the N6 medium (Fe2+ content is 5.6mg/kg) 1.4 times more, in this within, with Fe2+ content increase, the induced rate increase, more than this content, the induced rate induce, showed a single peak of distribution. When Fe2+ content in differentiation medium increased to 40mg/kg, the differentiation rate was significantlylower. But the rate of green plant on the differentiation of callus was obvious differences in MS medium, which was induced in the medium of 32-40 mg/kg Fe2+ content, about 71.4% of materials can improve the efficiency of anther culture to 9.2%.So, we consider that increasing the Fe2+ content in induced medium can increase anther culture efficiency of early japonica in cold region. |
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Keywords: | Key words: early japonica in cold regionzz anther culturezz mediumzz iron effectzz |
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