福建黄兔微卫星标记与生长性状的相关分析

根据兔的遗传图谱及相关报道选择了8个微卫星基因座对195只福建黄兔群体进行群体遗传学检测;计算其有效等位基因数杂合度、多态信息含量,并通过最小二乘分析微卫星遗传标记与体重、体尺性状的相关性.结果表明选用的8个微卫星座位的杂合度在0.5904～0.7267之间,平均为0.6857.多态信息含量在0.5723～0.7222之间,平均为0.6622.许多微卫星基因型与体尺体重有较强相关.微卫星座位Sol133 209bp等位基因对1月龄体重有较强的相关效应;Sat12 125bp和座位So133 201bp等2个等位基因对3月龄体重有较强的相关效应;座位So133 213bp等位基因对8月龄体重有负面影响;座位So144 217bp、座位So133 209bp、以及座位So103 236bp等4个等位基因对体长性状有相关效应;座位So144 227bp、座位So133 209bp、座位So130 170bp以及座位Sp103 244bp等4个位基因对胸围性状有相关效应;座位So144 203bp等位基因对胸围性状有负面影响.5个微卫星基因座的基因型So133(209/213)、Sat12(125)、So130 170、So103(236/244)、So144(203/227)对福建黄兔体尺体重存在相关性.这为进一步OTL定位提供理论依据.

Correlation Analysis between Microsatellite Marks and Growth Traits in Fujian Yellow Rabbit

According to the rabbit linkage map and reports, 8 microsatellite loci were selected to detect the genetic polymorphism in 195 Fujian yellow rabbits.Effective number of alleles, heterozygositry, polymor-phism information content, and correlation analysis between microsatellite marks and body weight as well were calculated by least square means.Results were showed as follow:The average heterozygosit(H) was 0.6857,ranging 0.5904 from 0.7267.The average polymorphism information content(PIC) was 0.6622, rang-ing from 0.5723 to 0.7222.Many genotypes of microsatellites were significant correlations with body size and body weight.The allele 209bp of Sol33 had a positive effect on first month''s weight.The allele 125bp of Sat12 and allele 201bp of Sol33 had a strong positive impact on third 1-month-old weight.The allele 213bp of Sol33 may have a negative impact on the 8-month-old weight.If allele 242bp of Sol03 had a positive im-pact, the allele 240bp had a strong negative impact on the 8-month-old weight.The allele 217bp of Sol44, allele 209bp of Sol33, allele 153bp of Sol30 and allele 236bp of Sol03 had a positive impact on stem length.The allele 209bp of Sol33, allele 170bp of Sol30 and allele 244bp of Sol03 may have a positive impact on chest circumference.The allele 203bp of Sol44 may have a negative impact on chest circumference.The five microsatellites genotypes of Sol33(209/213), Sat12(125), Sol30 170, Sol03(236/244), Sol44(203/227) had a significant correlation with weight and body size.This will provide a theoretical basis for further QTL.