| 外源ABA和乙烯利胁迫下斑茅ACO基因表达的实时PCR分析 |
| |
| 作者姓名： | 郭春芳 张积森 张木清 陈如凯 |
| |
| 作者单位： | [1]福建农林大学农业部甘蔗生理生态与遗传改良重点实验室,福州350002 [2]福建教育学院,福州350025 [3]福建师范大学生命科学学院,福州350108 |
| |
| 基金项目： | 国家自然科学基金
,
高等学校博士学科点专项科研项目 |
| |
| 摘 要： | 研究了外源ABA和乙烯利胁迫处理对斑茅1-氨基环丙烷-1-羧酸氧化酶基因（ACO基因）表达情况的影响。在项目组克隆斑茅ACO基因的基础上，应用实时荧光PCR技术分析斑茅ACO基因在ABA、乙烯利胁迫下的表达。结果表明，斑茅ACO基因在ABA的胁迫下，在3h有微弱上调表达，而6h日寸明显抑制，其后表达趋向平缓；在乙烯利的胁迫下，ACO基因在前期受抑制，其后呈明显上调表达，在24h后ACO基因的表达趋向平缓。斑茅ACO基因的表达受乙烯利诱导，而不受外源ABA诱导。
|
| 关 键 词： | 斑茅 1-氨基环丙烷-1-羧酸氧化酶基因(ACO 基因) 脱落酸 乙烯利 实时荧光PCR 乙烯利 胁迫处理 斑茅 基因表达 技术分析 Realtime Stress Ethephon Exogenous ABA Gene Expression 平缓 上调表达 结果 实时荧光 应用 克隆 项目组 影响 情况 |
Analysis of The Expression of ACO Gene Under Exogenous ABA and Ethephon Stress in Erianthus arundinaceus throught Realtime PCR |
| |
| Authors: | Guo Chunfang Zhang Jisen Zhang Muqing Chen Rukai |
| |
| Abstract: | In order to study the molecular mechanism of A CO gene from Erianthus arundinaceum under exogenous ABA and ethephon stress. In this paper, based on the cloning of A CO gene in Erianthus arundi- naceus, the expression of A CO gene in Erianthus arundinaceus under ABA and ethephon treatments was investigated by Real-time fluorescence PCR. The results showed that the expression of A CO gene was slightly up-regulated at the beginning and continuous inhibited, then became normal by ABA stress . Under the treatment of ethephon, the expression of A CO gene was prohibited at the earlier stage along with the subsequent continuous more obvious up-regulation. The results showed that the expression of A CO gene in Erianthus arundinaceus could be induced by ethephon, but not by ABA. |
| |
| Keywords: | |
|
| 点击此处可从《》浏览原始摘要信息 |
| 点击此处可从《》下载免费的PDF全文 |
