PCR及核酸探针检测耐甲氧西林葡萄球菌mecA基因的研究

为了解耐甲氧西林葡萄球菌在畜禽间的存在情况.试验建立针对mecA基因的PCR方法及地高辛标记核酸探针技术,分别用两种方法对75株葡萄球菌分离株进行检测.结果显示,经PCR方法检出42株耐甲氧西林葡萄球菌,占分离株的56.0%;经核酸探针方法检出47株耐甲氧西林葡萄球菌,占分离株的62.7%,两种方法的总符合率为93.3%.通过研究证实,在畜禽的体表及体内能够分离到耐甲氧西林葡萄球菌,且有较高的分离率,应该引起兽医工作者的足够重视.

Detection of mecA Gene of Methicillin-resistant Staphylococcus by PCR and Nucleic Acid Probe

In order to investigate the situation of methicillin-resistant Staphylococcus(MRS) in livestock and poultry, the method of PCR and dig-labeled nucleic acid probe to mecA gene fragment were established to detect 75 Staphylococcus isolates. Forty-two strains of MRS were screened out by PCR, accounting for 56.0%. Forty-seven strains of MRS were screened out by nucleic acid probe method, accounting for 62.7%. The coincidence rate of acid probe method with PCR method was 93.3%. It was verified that MRS could be isolated from livestock and poultry in vivo or in vitro, with higher isolation rate. Veterinary researchers should pay more attention to Methicillin-resistant Staphylococcus.