| 蒜氨酸酶动力学性质研究 |
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| 引用本文： | 葛艳辉,赵俊英,闵笛,冯炘.蒜氨酸酶动力学性质研究[J].农业科学与技术,2008,9(1):139-142. |
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| 作者姓名： | 葛艳辉 赵俊英 闵笛 冯炘 |
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| 作者单位： | [1]天津理工大学环境科学与安全工程学院,天津300191 [2]天津大学环境科学与工程学院,天津300071 |
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| 基金项目： | 天津市科委资助项目
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天津市高等学校科技发展基金 |
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| 摘 要： | 大蒜中含有一类被称之为大蒜素的含硫化合物，主要由蒜氨酸酶与蒜氨酸发生酶解反应生成，但蒜氨酸酶含量低，对环境较敏感，因而活性表现不稳定，加之目前的生产条件常导致该酶活性被破坏，使得蒜类产品的药理作用不显著。因此，研究蒜氨酸酶的理化性质对于开发应用蒜类药用产品具有重要的指导作用。
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| 关 键 词： | 蒜氨酸酶 动力学性质 大蒜素 活性 药理作用 |
Study on Kinetic Characteristics of Alliinase |
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| GE Yan-hui,ZHAO Jun-ying,MIN Di,FENG Xin.Study on Kinetic Characteristics of Alliinase[J].Agricultural Science ＆ Technology,2008,9(1):139-142. |
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| Authors: | GE Yan-hui ZHAO Jun-ying MIN Di FENG Xin |
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| Abstract: | Objective]The kinetic characteristics of alliinase was studied to select the optimum reaction performance.Method]Alliinase activity was measured to analysis the influence of temperature,pH,substrate concentration and metal iron.Result]Alliinsse was an enzyme with thermal instability.Its optimum reaction temperature was 29℃ and pH value was 6.1.The Vmax was 0.439 IU/mg and Km was 0.483 mmol/L by using natural extract as substrate.Alliinase activity was activated when the K ,Mg2 ,Na and CA2 existed and alliinase activity was inhibited when Cu2 existed.Concluslon]Results showed that the kinetic characteristics of alliinase supply the academic foundation for development and application of garlic medical products. |
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| Keywords: | Allium sativum Alliinase Kinetic characteristics |
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