|
|||||
|
|
In vitro and in vivo effects of some pesticides on carbonic anhydrase enzyme from rainbow trout (Oncorhynchus mykiss) gills |
|
| Authors: | Saltuk Bu?rahan Ceyhun,Murat ?entü rk,Orhan Erdo?an,Ö mer ?rfan Kü frevio?lu |
| Affiliation: | a Atatürk University, H?n?s Vocational Training School, 25600 Erzurum, Turkey b Atatürk University, Biotechnology Research Center, 25240 Erzurum, Turkey c A?r? ?brahim Çeçen University, Art and Science Faculty, Chemistry Department, 04100 A?r?, Turkey d Atatürk University, Science Faculty, Chemistry Department, 25240 Erzurum, Turkey e Atatürk University, Agriculture Faculty, Aquaculture Department, 25240 Erzurum, Turkey |
| Abstract: | Here we investigated the in vitro and in vivo effects of the pesticides, deltamethrin, diazinon, propoxur and cypermethrin, on the activity of rainbow trout (rt) gill carbonic anhydrase (CA). The enzyme was purified from rainbow trout gills using Sepharose 4B-aniline-sulfanilamide affinity chromatography method. The overall purification was approx. 214-fold. SDS-polyacrylamide gel electrophoresis showed a single band corresponding to a molecular weight of approx. 29 kDa. The four pesticides dose-dependently inhibited in vitro CA activity. IC50 values for deltamethrin, diazinon, propoxur and cypermethrin were 0.137, 0.267, 0.420 and 0.460 μM, respectively. In vitro results showed that pesticides inhibit rtCA activity with rank order of deltamethrin > diazinon > propoxur > cypermethrin. Besides, in vivo studies of deltamethrin were performed on CA activity of rainbow trout gill. rtCA was significantly inhibited at three concentrations (0.25, 1.0 and 2.5 μg/L) at 24 and 48 h. |
| Keywords: | Carbonic anhydrase Pesticide Deltamethrin Oncorhynchus mykiss Gill |
| 本文献已被 ScienceDirect 等数据库收录！ | |