Influences of sub-acute exposure to paraquat on cytochrome P450 3A2 expression in rat liver and lungs |
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Authors: | H Malekinejad F Rahmani |
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Institution: | a Department of Pharmacology & Toxicology, Faculty of Veterinary Medicine, P.O. Box: 1177, Urmia University, Urmia, Iran b Department of Molecular Genetic, Faculty of Basic Sciences, Urmia University, Urmia, Iran |
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Abstract: | This study was designed to investigate the possible effects of paraquat sub-acute poisoning on cytochrome P450 3A2 expression in the liver and lungs. Twenty adult male rats (150-200 g) were exposed either against saline normal as control group or various doses of paraquat (3.5, 7 and 10 mg/kg, s.c.) as test groups for 7 consecutive days. Paraquat-exposed animals showed loss of body weight and elevation in serum levels of alkaline phosphates and alanine aminotransferase in a dose-dependent fashion. Moreover, animals in the test groups demonstrated a significant (P < 0.05) increase of malondialdehyde content in both the liver and lung tissues. Ultimately, by using RT-PCR method it became clear that 7 days exposure to paraquat resulted in a total suppression of cytochrome P450 3A2 at the mRNA level in the lungs. By contrast, a considerable up regulation of the same gene occurred in the liver. This data suggest that paraquat not only affect the lungs as a main target tissue but also up regulates the predominant cytochrome P450 gene in the liver which may induce detoxification processes. |
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Keywords: | Paraquat Cytochrome P450 3A2 Liver Lung Lipid peroxidation |
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