| 甘露醇及AgNO_3对根癌农杆菌介导的多年生黑麦草遗传转化的影响 |
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| 引用本文： | 乔定君,毛萍,马欣荣,杨宏.甘露醇及AgNO_3对根癌农杆菌介导的多年生黑麦草遗传转化的影响[J].草业学报,2011,20(1). |
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| 作者姓名： | 乔定君 毛萍 马欣荣 杨宏 |
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| 作者单位： | 乔定君,毛萍,QIAO Ding-jun,MAO Ping(中国科学院成都生物研究所生态恢复重点实验室,四川,成都,610041;中国科学院研究生院,北京,100049);马欣荣,杨宏,MA Xin-rong,YANG Hong(中国科学院成都生物研究所生态恢复重点实验室,四川,成都,610041) |
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| 基金项目： | 国家863计划项目，中国科学院知识创薪工程重要方向项目 |
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| 摘 要： | 多年生黑麦草是优良的牧草和草坪草.农杆菌介导多年生黑麦草遗传转化非常困难.为了建立稳定的多年生黑麦草遗传转化体系,本研究以品种"多福"种子成熟胚为外植体诱导愈伤组织,携质粒pCAMBIA2301的根癌农杆菌EHA105介导,进行遗传转化.在预培养及共培养基中添加0.1 mol/L甘露醇或5 mg/L硝酸银,并分析了二者对转化的影响.经巴龙霉素筛选、PCR及GUS组织染色检测表明,共获得了转基因植株38株.添加O.1mol/L甘露醇或5 mg/L AgNO3或O.1 mol/L甘露醇+5 mg/L AgNO3,转化效率分别为对照的1.96,1.59和2.95倍.结果表明,在根癌农杆菌介导的多年生黑麦草愈伤组织遗传转化中,在预培养和共培养基中添加甘露醇或AgNO3能提高转化率,且当二者共同添加时可显著提高.
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| 关 键 词： | 多年生黑麦草 根癌农杆菌 遗传转化 愈伤组织 硝酸银 甘露醇 |
The effects of mannitol and AgNO_3 for perennial ryegrass transformation mediated by Agrobacterium tumefaciens |
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| QIAO Ding-jun,MAO Ping,MA Xin-rong,YANG Hong.The effects of mannitol and AgNO_3 for perennial ryegrass transformation mediated by Agrobacterium tumefaciens[J].Acta Prataculturae Sinica,2011,20(1). |
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| Authors: | QIAO Ding-jun MAO Ping MA Xin-rong YANG Hong |
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| Institution: | QIAO Ding-jun1,2,MAO Ping1,MA Xin-rong1,YANG Hong1(1.ECORES Lab,Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,China,2.Graduate University of the Chinese Academy of Sciences,Beijing 100049,China) |
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| Abstract: | Perennial ryegrass(Lolium perenne),a graminaceous crop,is one of the most widely distributed grass in the temperate regions for forage and turf.However,its transformation mediated by Agrobacterium tumefaciens is largely recalcitrant and lagged.In this paper,an improved method for perennial ryegrass mediated by A.tumefaciens was developed.And the transgenic plants were successfully obtained.Embryogenic calli induced from mature embryos of a perennial ryegrass variety Tove were transformed by A.tumefaciens st... |
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| Keywords: | perennial ryegrass Agrobacterium tumefaciens genetic transformation callus induction silver nitrate mannitol |
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