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| 杜氏盐藻MAPK基因植物表达载体的构建及对烟草的转化 | |
| 引用本文： | 郭丽,段金秀,梁占训,孙晓玲,张鹤山,王小行,曹致中. 杜氏盐藻MAPK基因植物表达载体的构建及对烟草的转化[J]. 草原与草坪, 2006, 0(4): 32-36 |
| 作者姓名： | 郭丽 段金秀 梁占训 孙晓玲 张鹤山 王小行 曹致中 |
| 作者单位： | 1. 甘肃农业大学,草业学院,甘肃,兰州,730070 2. 甘肃格瑞生态技术有限公司,甘肃,兰州,730000 3. 四川川大光耀生物工程有限公司,四川,成都,610063 |
| 基金项目： | 国家高技术研究发展计划(863计划);科技部重大专项基金 |
| 摘 要： | 以载体pBI121为基础,经SmaI和BamHI单酶切后,构建了植物表达载体pBI121-MAPK,并通过农杆菌介导采用叶盘转化法将其转入烟草中,经卡那霉素抗性筛选,获得转基因抗性植株。经PCR检测表明pBI121-MAPK已整和到烟草基因组中。 |
| 关 键 词： | pBI121-MAPK 表达载体 农杆菌介导法 烟草 |
| 文章编号： | 1009-5500(2006)04-0032-05 |
| 修稿时间： | 2005-11-24 |
Plant expressing vector construction of MAPK gene in Dunaliella salina and transformation to tobacco |
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| GUO Li,DUAN Jin-xiu,LIANG Zhan-xun,SUN Xiao-ling,ZHANG He-shan,WANG Xiao-xing,CAO Zhi-zhong. Plant expressing vector construction of MAPK gene in Dunaliella salina and transformation to tobacco[J]. Grassland and Turf, 2006, 0(4): 32-36 | |
| Authors: | GUO Li DUAN Jin-xiu LIANG Zhan-xun SUN Xiao-ling ZHANG He-shan WANG Xiao-xing CAO Zhi-zhong |
| Abstract: | The plasmid pBI121 was cut by SmaI and BamHI,and then the plant expressing vector of pBI121-MAPK was successful constructed.Leaf segments of tobacco were infected by Agrobacterium tumefaciens EHA105 with plasmid pBI121-MAPK,from which,the kanamycin resistant translated plants were obtained.PCR analysis proved that the pBI121-MAPK gene was integrated into the genomes of the tobacco plants. |
| Keywords: | pBI121-MAPK expressing vector Agrobacterium tumefaciens mediation tobacco |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |