| 瑞昌山药组培快繁研究 |
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| 引用本文： | 彭琴,谢星文,李秋明.瑞昌山药组培快繁研究[J].安徽农业科学,2009,37(32):15713-15714. |
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| 作者姓名： | 彭琴 谢星文 李秋明 |
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| 作者单位： | 九江学院生命科学学院,江西九江,332000;九江学院生命科学学院,江西九江,332000;九江学院生命科学学院,江西九江,332000 |
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| 基金项目： | 九江学院重点资助项目 |
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| 摘 要： | 目的]探讨瑞昌山药组培快繁的技术环节。方法]以瑞昌山药茎段为外植体，通过组织培养试验研究了不同激素及其浓度对愈伤组织形成、丛生芽诱导和生根的影响。结果]70％乙醇和0．1％HgCl2结合对外植体的的消毒效果最佳。培养基Ms＋6-BA2．0mg／L＋NAA0．2mg／L中的腋芽生长最好，培养基MS＋6一BA1．0mg／L＋NAA0．2mg／L、MS＋6一BA1．0mg／L-I-NAA0．5mg／L、MS＋6一BA2．0mg／L＋NAA0．2mg／L和MS＋6一BA2．0mg／L-I-NAA0．5mg／L中腋芽的诱导率分别为52％、43％、84％和48％。在继代培养中，采用原诱导培养基诱导丛生芽的效果较差，降低6一BA的浓度有利于丛生芽的诱导。培养基1／2MS＋IBA1．5mg／L＋NAA0．3mg／L4-活性炭0．2mg／L中幼苗的生根效果较好。结论]该研究为组织培养技术在种苗繁育中的应用奠定了基础。
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| 关 键 词： | 瑞昌山药 组织培养 愈伤组织 丛芽诱导 |
Research on the Tissue Culture and Rapid Propagation of Ruichang Yam |
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| PENG Qin et al.Research on the Tissue Culture and Rapid Propagation of Ruichang Yam[J].Journal of Anhui Agricultural Sciences,2009,37(32):15713-15714. |
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| Authors: | PENG Qin |
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| Institution: | PENG Qin et al (College of Life Science,Jiujiang University,Jiujiang,Jiangxi 332000) |
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| Abstract: | Objective]The aim of the research was to discuss the technical links in the tissue culture and rapid propagation of Ruichang yam.Method]With the stem segments of Ruichang yam as explants,the effects of different hormones and their concn.on callus formation,tufted-bud induction and rooting were researched through tissue culture experiments.Result]The disinfection efficacy of combing 70% alcohol with 0.1% HgCl_2 on the explants was best.The axillary buds grew best in the medium MS+6-BA 2.0 mg/L+NAA 0.2 mg/... |
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| Keywords: | Ruichang yam Tissue culture Callus Tufted-bud induction |
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