两个小麦抗赤霉变异系的抗性及RAPD分析

在禾谷镰刀菌粗毒素胁迫下，通过幼胚培养无性系的筛选，获得了QK-02和HK-04两个抗赤霉病的小麦变异系，与其供体亲本相比，抗赤性明显提高，其它个别性状虽略有改变，但其综合性状表现与其亲本基本相似。用524个随机引物对两个变异系和各自亲本及苏麦3号进行RAPD分析，结果显示，变异系QK-02与亲本西农1376间存在多态片段的引物有11个，变异率为2.1%，在这些多态片段中，S3471220和S3751360在苏麦3号上也能检测到；变异系HK-04与亲本花育888间有15条引物有多态片段，变异率为2.86%，在这些多态片段中，S149620在苏麦3号上也能检测到。RAPD分析结果表明，两个抗赤霉变异系与亲本的抗病性差异是由于遗传物质的改变引起的，其抗赤霉病性是可以稳定遗传的。在抗病变异系与亲本及苏麦3号上筛选到的3个分子标记（S3471220、S3751360和 S149620）可能与小麦赤霉病抗性有关。

Resistance and RAPD Analysis of Two Variant Lines with Resistant to Scab in Wheat

Two variant lines with resistant to scab in wheat,Qk-02 and Hk-04,were selected through multi-setp selection under treatment of the wheat scab pathogenic crude toxin in vitro culture of young embryo. Resistance to scab and agronomic traits of two variant lines were campared with that of their donors .The results showed that resistance to scab of two variant lines were improved obviously and integral traits were similar to that of their parents respectively.,although several agronomic traits have been changed. For verifing the variation, 524 random primers were used to amplify DNA from variant lines,their donors and Sumai 3. The results were as follows：①11 primers showed amplified product polymorphism in QK-02 variant line and its parent Xinong 1376, variation rate was 2.1%. Among them, two polymorphic DNA makers (S3471220、S3751360)were observed in Sumai 3;②15 primers showed amplified product polymorphism in HK-04 variant line and its parent Huayu 888,variation rate was 2.86%,one polymorphic DNA maker （S149620）was observed in Sumai 3,which reached the conclusion that variant lines of resistance to scab took place changes at the DNA level,and three RAPD makers（S3471220, S3751360, S149620）were related to scab resistance.