猪附红细胞体可溶性抗原的分析

为了解附红细胞体可溶性抗原的特性,本试验对解离下的猪附红细胞体,经超声波裂解制备粗抗原,再经SephadexG-200分离提纯后,应用对流免疫电泳(CIEP)定位特异性抗原蛋白峰,再进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)及免疫印迹(Westernblot),对猪附红细胞体粗提及纯化的可溶性抗原进行了分析。结果表明,猪附红细胞体可溶性抗原的电泳图谱上有四条蛋白带,其分子量分别为77Ku、62Ku、58Ku、29Ku,其中特异性抗原分子量为58Ku和29Ku。从而为附红细胞体抗原成分的进一步分析及该病的免疫学诊断等研究奠定了基础。

The analysis of soluble antigen of Eperythrozoon suis

In order to grasp soluble antigen trait of Eperythrozoon suis,we have analyzed crude and purified soluble antigen of Eperythrozoon suis using SDS-PAGE and Western-blotting.The preparation of crude antigen was that splited dissociated Eperythrozoon using ultrasonic wave.To prepare the purified antigens,we separated and purified crude antigen by Sephadex G-200,localizated characteristic antigen protein peak value by CIEP.The results showed that soluble antigen of Eperythrozoon suis contained four protein strips,its molecular weight were 77 Ku?62 Ku?58 Ku?29 Ku.Thereinto the molecular weight of characteristic soluble antigen was 58 Ku and 29 Ku.This test has established the foundation for analyzing antigen component of Eperythrozoon and immunology diagnoses of Eperythrozoonosis.