| 猪附红细胞体可溶性抗原的分析 |
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| 引用本文： | 贾立军,曲平,张守发,常巧呈.猪附红细胞体可溶性抗原的分析[J].中国预防兽医学报,2006,28(5):569-571. |
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| 作者姓名： | 贾立军 曲平 张守发 常巧呈 |
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| 作者单位： | 延边大学农学院动物医学系,吉林,龙井,133400 |
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| 摘 要： | 为了解附红细胞体可溶性抗原的特性,本试验对解离下的猪附红细胞体,经超声波裂解制备粗抗原,再经SephadexG-200分离提纯后,应用对流免疫电泳(CIEP)定位特异性抗原蛋白峰,再进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)及免疫印迹(Westernblot),对猪附红细胞体粗提及纯化的可溶性抗原进行了分析。结果表明,猪附红细胞体可溶性抗原的电泳图谱上有四条蛋白带,其分子量分别为77Ku、62Ku、58Ku、29Ku,其中特异性抗原分子量为58Ku和29Ku。从而为附红细胞体抗原成分的进一步分析及该病的免疫学诊断等研究奠定了基础。
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| 关 键 词： | 猪附红细胞体 可溶性抗原 免疫印迹 |
| 文章编号： | 1008-0589(2006)05-0569-03 |
| 收稿时间： | 2005-05-17 |
| 修稿时间： | 2005年5月17日 |
The analysis of soluble antigen of Eperythrozoon suis |
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| JIA Li-jun,QU Ping,ZHANG Shou-fa,CHANG Qiao-cheng.The analysis of soluble antigen of Eperythrozoon suis[J].Chinese Journal of Preventive Veterinary Medicine,2006,28(5):569-571. |
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| Authors: | JIA Li-jun QU Ping ZHANG Shou-fa CHANG Qiao-cheng |
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| Institution: | Agricultural College of Yanbian University, Longjing 133400, China |
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| Abstract: | In order to grasp soluble antigen trait of Eperythrozoon suis,we have analyzed crude and purified soluble antigen of Eperythrozoon suis using SDS-PAGE and Western-blotting.The preparation of crude antigen was that splited dissociated Eperythrozoon using ultrasonic wave.To prepare the purified antigens,we separated and purified crude antigen by Sephadex G-200,localizated characteristic antigen protein peak value by CIEP.The results showed that soluble antigen of Eperythrozoon suis contained four protein strips,its molecular weight were 77 Ku?62 Ku?58 Ku?29 Ku.Thereinto the molecular weight of characteristic soluble antigen was 58 Ku and 29 Ku.This test has established the foundation for analyzing antigen component of Eperythrozoon and immunology diagnoses of Eperythrozoonosis. |
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| Keywords: | SDS-PAGE |
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