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抗虫转基因欧洲黑杨的Western印迹法分析
引用本文:陈颖,田颖川.抗虫转基因欧洲黑杨的Western印迹法分析[J].林业科学,1996,32(3):274-276.
作者姓名:陈颖  田颖川
作者单位:中国林业科学研究院林业研究所,中国科学院微生物研究所
摘    要:抗虫转基因欧洲黑杨的Western印迹法分析陈颖,李强,李玲,韩一凡,田颖川(中国林业科学研究院林业研究所北京100091)(中国科学院微生物研究所北京100080)关键词Bt.基因,转基因欧洲黑杨,Western印迹法WESTERNBLOTANAL...

关 键 词:Bt.基因,转基因欧洲黑杨,Western印迹法

WESTERN BLOT ANALYSIS OF TRANSGENIC POPULUS NIGRA PLANTS TRANSFORMED WITH BACILLUS THURINGIENSIS TOXIN GENE
Chen Ying ,Li Qiang, Li Ling, Han Yifan.WESTERN BLOT ANALYSIS OF TRANSGENIC POPULUS NIGRA PLANTS TRANSFORMED WITH BACILLUS THURINGIENSIS TOXIN GENE[J].Scientia Silvae Sinicae,1996,32(3):274-276.
Authors:Chen Ying  Li Qiang  Li Ling  Han Yifan
Abstract:Four transgenic Populus nigra plants which were transformed with Bacillus thuringiensis toxin gene and showed high insect resistance were analysed by Western blot method. The result showed that 55kD polypeptides were found in the positive control and four transgenic plants showed Bt. toxin gene was not only inserted into the chromosomes of poplar, but also expressed into protein. The result gave a direct evidence to explain the four plants's high insect resistance.
Keywords:Bt  toxin gene  Transgenic Populus nigra  Western blot analysis  
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