| 弓形虫感染鼠脾细胞microRNA表达谱的检测分析 |
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| 引用本文： | 金洪涛,商立民,刘全. 弓形虫感染鼠脾细胞microRNA表达谱的检测分析[J]. 中国兽医学报, 2012, 32(2): 252-257 |
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| 作者姓名： | 金洪涛 商立民 刘全 |
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| 作者单位： | 军事医学科学院军事兽医研究所吉林省人兽共患病预防与控制重点实验室,吉林长春,130062 |
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| 基金项目： | 国家自然科学基金资助项目(30972178,31001057);国家科技支撑计划资助项目(2010BAD04B01) |
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| 摘 要： | MicroRNA（miRNA）是一类约22个核苷酸组成的单链非编码RNA,在分化、发育、肿瘤形成等方面起着重要作用。本研究对弓形虫RH株感染小鼠脾细胞miRNA的表达进行了特异性基因芯片检测分析,并应用荧光定量RT-PCR方法进行验证。结果表明,感染鼠脾细胞中与免疫应答及细胞增殖和肿瘤发生相关的三大类miRNA中,有39种表达下调,同时有36种表达上调。上述结果揭示,弓形虫感染机体后伴随着靶细胞功能性miRNA表达谱的显著改变,这为进一步研究弓形虫感染致病的分子机制开辟了新的方向。
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| 关 键 词： | 弓形虫 microRNA 基因芯片 荧光定量RT-PCR |
Altered splenic microRNA expression profile in mice infected with Toxoplasma gondii RH strain |
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| JIN Hong-tao,SHANG Li-min,LIU Quan. Altered splenic microRNA expression profile in mice infected with Toxoplasma gondii RH strain[J]. Chinese Journal of Veterinary Science, 2012, 32(2): 252-257 |
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| Authors: | JIN Hong-tao SHANG Li-min LIU Quan |
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| Affiliation: | *(Laboratory of Parasitology,Institute of Military Veterinary,AMMS / Key Laboratory of Jilin Province for Zoonosis Prevention and Control,Changchun 130062,China) |
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| Abstract: | MicroRNAs(miRNAs) are a new class of endogenous non-coding RNAs that play critical roles in a wide spectrum of biological processes.In this study,splenic miRNA expression in mice infected with Toxoplasma gondii RH strain was analyzed using locked nucleic acid-based miRNA arrays,and further validated by quantitative realtime RT-PCR.The results indicated that there were 39downregulated miRNAs and 36upregulated miRNAs,which were classified into three functional groups relative to immune response,cell proliferation and cancer.The preliminary functional analysis of altered miRNA expression suggested that miRNAs are involved in pathogenesis of T.gondii infection,which may provide a new method to analyze molecular mechanisms of toxoplasmosis. |
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| Keywords: | Toxoplasma gondii microRNA microarray quantitative real-time RT-PCR |
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