| 溴氰菊酯对克氏原螯虾的氧化胁迫效应 |
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| 引用本文： | 魏华,吴楠,沈竑,成永旭,吴婷婷. 溴氰菊酯对克氏原螯虾的氧化胁迫效应[J]. 水产学报, 2010, 34(5): 733-739. DOI: 10.3724/SP.J.1231.2010.06709 |
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| 作者姓名： | 魏华 吴楠 沈竑 成永旭 吴婷婷 |
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| 作者单位： | 1. 上海海洋大学水产与生命学院,上海,201306
2. 上海滩涂生物资源开发研究所,上海,202150 |
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| 基金项目： | 上海市科委攻关项目(08dz1206002) |
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| 摘 要： | 为了解溴氰菊酯对克氏原螯虾的毒性及致毒机理,采用24h换水式生物试验研究了溴氰菊酯对克氏原螯虾的96h急性毒性,分光光度法检测了6、12、24和48h后0.01、0.02和0.04μg/L溴氰菊酯对肝胰腺超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活力和丙二醛(MDA)的含量等氧化胁迫相关指标的影响。结果表明,24、48和96h的半致死浓度分别为0.1560、0.0993和0.0562μg/L,安全浓度为5.62ng/L;在整个暴露过程中,溴氰菊酯各个处理组都引起了氧化胁迫相关指标的变化。SOD和CAT活力的变化趋势相同,都呈抑制-诱导-抑制的变化规律,MDA含量则一直高于对照组。暴露6h后,0.01μg/L浓度组MDA含量极显著高于对照组(P<0.01),0.04μg/L浓度组MDA含量约为对照组的1.98倍(P<0.05);暴露12h后,MDA仍保持较高水平,0.02μg/L浓度组MDA含量约为对照组的1.76倍(P<0.05);暴露24h后,各浓度组CAT活力分别比对照组上升了70.98%、73.05%和66.67%(P<0.01);暴露48h后,0.01、0.02μg/L浓度组的SOD活力分别下降了...
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| 关 键 词： | 克氏原螯虾 溴氰菊酯 急性毒性 超氧化物歧化酶 过氧化氢酶 丙二醛 |
| 收稿时间： | 2009-11-16 |
| 修稿时间： | 2010-01-12 |
Oxidative stress of deltamethrin to the liver of crayfish Procambarus clarkii |
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| WEI Hu,WU Nan,SHEN Hong,CHEN Yong-xu and WU Ting-ting. Oxidative stress of deltamethrin to the liver of crayfish Procambarus clarkii[J]. Journal of Fisheries of China, 2010, 34(5): 733-739. DOI: 10.3724/SP.J.1231.2010.06709 |
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| Authors: | WEI Hu WU Nan SHEN Hong CHEN Yong-xu WU Ting-ting |
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| Affiliation: | WEI Hua1*,WU Nan1,SHEN Hong2,CHENG Yong-xu1,WU Ting-ting1 (1. College of Fisheries , Life Science,Shanghai Ocean University,Shanghai 201306,China,2. Shanghai Mudbeach Institute of Biological Resource Exploitation,Shanghai 202150,China) |
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| Abstract: | Deltamethrin ( DLM),one of the regular pyrethroid pesticides,has toxical effects on aquatic animals after entering the natural water due to human activities. As a large-sized crustacean species,crayfish (Procambarus clarkii) is an important link of eco-system of the water. In order to understand the toxical effects and mechanism of DLM on P. clarkii,an acute semi-static toxic test was carried out,and the oxidative stress relative indicators [activities of superoxide dismutase (SOD),catalase (CAT) and concen... |
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| Keywords: | crayfish ( Procambarus clarkii) deltamethrin ( DLM) acute toxity superoxide dismutase (SOD) catalase(CAT) malondialdehyde(MDA) |
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