禽类DNA疫苗研究进展

新型疫苗的研究对于禽类传染病的防制意义重大。传统疫苗是基于抗原刺激机体产生特异性抗体的原理,它们大多数激发机体的体液免疫,很难启动细胞免疫。脱氧核糖核酸(DNA)疫苗作为第3代疫苗,具备传统疫苗和其它基因工程苗不可比拟的优点,能够诱导全方位的免疫反应且使用更安全更方便,DNA疫苗是将外源基因与真核质粒重组后直接导入细胞内,使外源基因在宿主细胞内表达合成保护性抗原蛋白。这是模拟病毒自然感染提呈过程,既能产生细胞免疫,又能产生体液免疫。文章对DNA疫苗在禽类应用的可行性和应用研究新进展作了综述。

Advance in Avian DNA Vaccination

DNA vaccines were introduced less than a decade ago but have already been applied to a wide range of infectious and malignant diseases. DNA vaccination has become the fastest growing field in vaccine technology following reports at the beginning of the 90's that plasmid DNA induces an immune response to the plasmid-encoded antigen. Inoculation of naked DNA represents a novel approach to vaccine and immune therapeutic development. DNA vaccines or genetic immunization offers several advantages over the conventional vaccines for specific immune activation. Although a large number of vaccines have been made and are being used in the poultry industry, there have been no major advances in vaccine technology for this animal industry sector for decades. The potential advantages of DNA vaccines, such as over coming maternal immunity, in ovo delivery and absence of requirement for a cold-chain, combined with immunological efficacy make this new vaccine technology very attractive for the poultry industry. Here we review the published reports of experimental DNA vaccines developed for use in poultry and focuses on the trends, potentials and remaining barriers in the development of this new revolution in poultry vaccinology.