UV-B辐射处理小麦苗期叶片的蛋白质组分析

本研究以小麦品种聊大1号为试验材料,研究UV-B辐射对聊大1号苗期叶片蛋白质组的影响。双向电泳分析发现,UV-B辐射处理和未处理的聊大1号有15个蛋白差异点,经质谱分析成功鉴定出6个蛋白差异点:其中1个为Rubisco大亚基(碳水化合物代谢);2个为参与翻译类的蛋白;2个为具有运输类功能的蛋白;1个为质子-ATP酶α亚基。这些结果可为小麦抗UV-B辐射机理研究和抗性育种提供依据。

Analysis of wheat seeding leaf proteome after UV-B radiation

In order to investigate the results of UV-B radiation on the accumulation of proteins in wheat seeding leaves,the cultivar LiaoDa 1 was subjected to UV-B radiation stress.Compared with control samples,the UV-B radiation stressed samples accumulated significantly higher amounts of protein.These proteins were separated by two-dimensional electrophoresis(2-DE),visualized by silver staining and analyzed by Image Master.It revealed 15 differentially expressed polypeptides,6 of which were successful identified by MALDI-TOF,including a Rubisco large subunit,an ATP synthase CF1 alpha chain,proteins involved in protein translation and transportation.These results might provide information for further UV-B radiation stress study in wheat.